Igwe O J, Que Hee S S, Wagner W D
Fundam Appl Toxicol. 1986 May;6(4):733-46. doi: 10.1016/0272-0590(86)90186-7.
Inhalation and intraperitoneal (ip) studies with 1,2-dichloroethane (1,2-DCE) using Sprague-Dawley rats fed 0.15% disulfiram (tetraethylthiuram disulfide; Antabuse; DSF) in AIN-76 diet [approx. (79 +/- 11) mg DSF/kg body wt/day] resulted in the observation of a toxic interaction relative to either agent alone. The combination treatment caused testicular atrophy and histopathology in the liver and testes at 1,2-DCE inhalation concentrations greater than 300 ppm (an estimated dose of 194 mg/kg body wt/day) administered 5 days/week for 30 days or at ip doses of 150 mg 1,2-DCE/kg body wt/day over 30 days, 7 days/week. DSF lowered the 1,2-DCE dose at which liver enlargement, decreased liver/body weight ratios, and decreased body weight gains appeared relative to exposure to the 1,2-DCE alone. For the ip study, a decrease in spleen weight also occurred. Testicular atrophy and liver pathology had previously been observed in rats exposed to 20 ppm of ethylene dibromide (EDB) and fed 0.05% DSF in rat chow diet; EDB or DSF alone did not elicit testicular atrophy. These toxic effects indicate that 1,2-DCE may interact with DSF as does EDB, but at a much higher dose.
使用Sprague-Dawley大鼠进行的1,2-二氯乙烷（1,2-DCE）吸入和腹腔注射（ip）研究中，给大鼠喂食AIN-76饮食中含0.15%双硫仑（四乙基秋兰姆二硫化物；戒酒硫；DSF）[约（79±11）mg DSF/千克体重/天]，结果观察到相对于单独使用任一药物存在毒性相互作用。联合治疗在1,2-DCE吸入浓度大于300 ppm（估计剂量为194 mg/千克体重/天）、每周给药5天、持续30天，或腹腔注射剂量为150 mg 1,2-DCE/千克体重/天、每周7天、持续30天时，会导致睾丸萎缩以及肝脏和睾丸出现组织病理学变化。与单独接触1,2-DCE相比，DSF降低了出现肝脏肿大、肝脏/体重比降低和体重增加减少的1,2-DCE剂量。对于腹腔注射研究，脾脏重量也出现了下降。此前在暴露于20 ppm二溴乙烷（EDB）并在大鼠饲料中喂食0.05% DSF的大鼠中观察到睾丸萎缩和肝脏病变；单独使用EDB或DSF不会引发睾丸萎缩。这些毒性作用表明，1,2-DCE可能与DSF发生相互作用，就像EDB一样，但剂量要高得多。
