Instituto de Ciências Moleculares/Centro de Investigação em Química da Universidade do Porto (IMS/CIQUP), Faculdade de Ciências da Universidade do Porto, Departamento de Química e Bioquímica, Rua do Campo Alegre 687, 4169-007, Porto, Portugal.
REQUIMTE-LAQV/Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, Porto, Portugal.
Anal Chim Acta. 2023 Jun 8;1259:341168. doi: 10.1016/j.aca.2023.341168. Epub 2023 Apr 2.
A plasmonic nanostructure was constructed as a biorecognition element coupled to an optical sensing platform in sandwich format, targeting the hazelnut Cor a 14 allergen-encoding gene. The analytical performance of the genosensor presented a linear dynamic range between 100 amol L and 1 nmol L, a limit of detection (LOD) < 19.9 amol L, and a sensitivity of 13.4 ± 0.6 m°. The genosensor was successfully hybridized with hazelnut PCR products, tested with model foods, and further validated by real-time PCR. It reached a LOD <0.001% (10 mg kg) of hazelnut in wheat material (corresponding to 1.6 mg kg of protein) and a sensitivity of -17.2 ± 0.5 m° for a linear range of 0.001%-1%. Herein, a new genosensing approach is proposed as a highly sensitive and specific alternative tool with potential application in monitoring hazelnut as an allergenic food, protecting the health of sensitized/allergic individuals.
构建了等离子体纳米结构作为生物识别元件，与光学传感平台以三明治的形式偶联，以榛子 Cor a 14 过敏原编码基因作为检测目标。该基因传感器具有 100 amol L 至 1 nmol L 的线性动态范围、< 19.9 amol L 的检测限 (LOD) 和 13.4 ± 0.6 m°的灵敏度。该基因传感器与榛子 PCR 产物成功杂交，用模式食品进行了测试，并通过实时 PCR 进一步验证。它在小麦材料中达到了榛子的 LOD < 0.001%（10 mg kg）（相当于 1.6 mg kg 的蛋白质），并且在 0.001% 至 1%的线性范围内的灵敏度为-17.2 ± 0.5 m°。本文提出了一种新的基因传感方法，作为一种高灵敏度和特异性的替代工具，具有在监测作为过敏原性食物的榛子、保护致敏/过敏个体健康方面的潜在应用。
