Division of Otolaryngology, Tohoku Medical and Pharmaceutical University Hospital.
Department of Otolaryngology-Head and Neck Surgery, Tohoku University Graduate School of Medicine.
Tohoku J Exp Med. 2023 Jul 11;260(3):223-230. doi: 10.1620/tjem.2023.J035. Epub 2023 Apr 27.
Long-term voice abuse or sudden vocal fold microvascular disruption may lead to injury and subsequent repair/remodeling in the vocal fold mucosa. Periostin is known to be involved in airway remodeling and also in various otolaryngological diseases. D-β-aspartic acid is the major isomer of D-aspartic acid found in elderly tissue. In this study we investigated the expression and the role of D-β-aspartic acid and periostin in the formation of vocal fold polyps. The expression patterns of D-β-aspartic acid and periostin in 36 surgical specimens of vocal fold polyps from 36 patients were investigated immunohistochemically. In the epithelium of vocal polyps, D-β-aspartic acid was expressed in all cases. Expression of D-β-aspartic acid was detected in 25 samples obtained from patients with vocal fold polyps stroma. Expression of periostin was detected in 28 samples obtained from patients with vocal fold polyps. Two patterns of D-β-aspartic acid expression were observed in vocal fold polyps stroma: positive type and negative type. The following four patterns of periostin expression were observed in vocal fold polyps: negative type, superficial type, infiltrative type, and diffuse type. An association was observed between D-β-aspartic acid expression patterns and periostin expression patterns. From these findings we speculate that periostin and D-β-aspartic acid participate in certain pathological changes in vocal fold polyps, such as extracellular matrix accumulation, local fibrosis, and the formation and development of vocal fold polyps.
长期的声带滥用或声带微小血管突然破裂可能导致声带黏膜损伤和随后的修复/重塑。骨膜蛋白已知参与气道重塑，也参与各种耳鼻喉疾病。D-β-天冬氨酸是在老年组织中发现的 D-天冬氨酸的主要异构体。在这项研究中，我们研究了 D-β-天冬氨酸和骨膜蛋白在声带息肉形成中的表达和作用。通过免疫组织化学方法研究了 36 例声带息肉患者的 36 个手术标本中 D-β-天冬氨酸和骨膜蛋白的表达模式。在声带息肉的上皮中，所有病例均表达 D-β-天冬氨酸。在 25 例声带息肉基质中获得的标本中检测到 D-β-天冬氨酸的表达。在 28 例声带息肉患者的标本中检测到骨膜蛋白的表达。在声带息肉基质中观察到两种 D-β-天冬氨酸表达模式：阳性型和阴性型。在声带息肉中观察到四种骨膜蛋白表达模式：阴性型、浅表型、浸润型和弥漫型。D-β-天冬氨酸表达模式与骨膜蛋白表达模式之间存在相关性。根据这些发现，我们推测骨膜蛋白和 D-β-天冬氨酸参与了声带息肉的某些病理变化，如细胞外基质积累、局部纤维化和声带息肉的形成和发展。
