Medical School of Ningbo University, Ningbo, Zhejiang, China.
Jingzhou Hospital Affiliated to Yangtze University, Jingzhou, Hubei, China.
BMC Pediatr. 2023 Apr 26;23(1):197. doi: 10.1186/s12887-023-04020-2.
We investigated changes in plasma transfer RNA related fragments (tRF) in children with obstructive sleep apnea-hypopnea syndrome (OSAHS) and the potential value as a disease marker.
Firstly, we randomly selected five plasma samples from the case group and the control group for high-throughput RNA sequencing. Secondly, we screened one tRF with different expression between the two groups, amplified it by quantitative reverse transcription-PCR (qRT-PCR) and sequenced the amplified product. After confirming that the qRT-PCR results were consistent with the sequencing results and the sequence of the amplified product contained the original sequence of the tRF, we performed qRT-PCR on all samples. Then we analyzed the diagnostic value of the tRF and its correlation with some clinical data.
A total of 50 OSAHS children and 38 control children were included in this study. There were significant differences in height, serum creatinine (SCR) and total cholesterol (TC) between the two groups. The plasma expression levels of tRF-21-U0EZY9X1B (tRF-21) were significantly different between the two groups. Receiver operating characteristic curve (ROC) showed that it had valuable diagnostic index, with area under the curve (AUC) of 0.773, 86.71% and 63.16% sensitivity and specificity.
The expression levels of tRF-21 in the plasma of OSAHS children decreased significantly which were closely related to hemoglobin, mean corpuscular hemoglobin, triglyceride and creatine kinase-MB, may become novel biomarkers for the diagnosis of pediatric OSAHS.
我们研究了阻塞性睡眠呼吸暂停低通气综合征（OSAHS）患儿血浆转移 RNA 相关片段（tRF）的变化及其作为疾病标志物的潜在价值。
首先，我们从病例组和对照组中随机选择了五个血浆样本进行高通量 RNA 测序。其次，我们筛选出两组之间表达差异的一个 tRF，通过定量逆转录-PCR（qRT-PCR）扩增，并对扩增产物进行测序。在确认 qRT-PCR 结果与测序结果一致且扩增产物的序列包含 tRF 的原始序列后，我们对所有样本进行 qRT-PCR。然后，我们分析了 tRF 的诊断价值及其与一些临床数据的相关性。
本研究共纳入 50 例 OSAHS 患儿和 38 例对照组儿童。两组间身高、血清肌酐（SCR）和总胆固醇（TC）存在显著差异。两组间 tRF-21-U0EZY9X1B（tRF-21）的血浆表达水平差异显著。受试者工作特征曲线（ROC）显示其具有有价值的诊断指标，曲线下面积（AUC）为 0.773，灵敏度和特异性分别为 86.71%和 63.16%。
OSAHS 患儿血浆中 tRF-21 的表达水平显著降低，与血红蛋白、平均红细胞血红蛋白、甘油三酯和肌酸激酶同工酶-MB 密切相关，可能成为小儿 OSAHS 诊断的新型生物标志物。
