miR-499血浆水平作为阻塞性睡眠呼吸暂停综合征生物标志物的潜力。
The potential of miR-499 plasmatic level as a biomarker of obstructive sleep apnea syndrome.
作者信息
Slouka David, Windrichova Jindra, Rezackova Hana, Houfkova Katerina, Kucera Radek, Cerna Vaclava, Kostlivy Tomas, Topolcan Ondrej, Pesta Martin
机构信息
Department of Otorhinolaryngology, University Hospital in Pilsen & Charles University, Faculty of Medicine in Pilsen, Edvarda Benese 13, 30599, Pilsen, Czech Republic.
Department of Immunochemistry Diagnostics, University Hospital in Pilsen & Charles University, Faculty of Medicine in Pilsen, Edvarda Benese 13, 30599, Pilsen, Czech Republic.
出版信息
Biomark Med. 2021 Aug;15(12):1011-1019. doi: 10.2217/bmm-2020-0826. Epub 2021 Jul 22.
Obstructive sleep apnea syndrome (OSAS) is one of the most common sleep-related breathing disorders. The aim of this study was to improve diagnostics in OSAS using blood circulating biomarkers. We consider the potential of cardiac-specific miRNAs in the diagnosis and risk assessment of cardiovascular complications. Plasmatic levels of miR-1-3p, miR-133a-3p and miR-499a-5p were measured by reverse transcription-PCR and compared with the clinical status of OSAS patients and controls. The level of miR-499 was higher (p = 0.0343) in OSAS patients (mean expression: 0.00561) compared with the controls (mean expression: 0.00003), using the multivariate logistic regression. The role of miR-499 in gene expression regulation during hypoxia and our findings indicate that miR-499 could be a new diagnostic biomarker for OSAS.
阻塞性睡眠呼吸暂停综合征（OSAS）是最常见的与睡眠相关的呼吸障碍之一。本研究的目的是利用血液循环生物标志物改善OSAS的诊断。我们考虑了心脏特异性微小RNA在心血管并发症诊断和风险评估中的潜力。通过逆转录聚合酶链反应测定了miR-1-3p、miR-133a-3p和miR-499a-5p的血浆水平，并与OSAS患者和对照组的临床状况进行了比较。使用多变量逻辑回归分析，与对照组（平均表达量：0.00003）相比，OSAS患者（平均表达量：0.00561）中miR-499的水平更高（p = 0.0343）。miR-499在缺氧过程中基因表达调控中的作用以及我们的研究结果表明，miR-499可能是OSAS的一种新的诊断生物标志物。
Zotero 插件