Slouka David, Windrichova Jindra, Rezackova Hana, Houfkova Katerina, Kucera Radek, Cerna Vaclava, Kostlivy Tomas, Topolcan Ondrej, Pesta Martin
Department of Otorhinolaryngology, University Hospital in Pilsen & Charles University, Faculty of Medicine in Pilsen, Edvarda Benese 13, 30599, Pilsen, Czech Republic.
Department of Immunochemistry Diagnostics, University Hospital in Pilsen & Charles University, Faculty of Medicine in Pilsen, Edvarda Benese 13, 30599, Pilsen, Czech Republic.
Biomark Med. 2021 Aug;15(12):1011-1019. doi: 10.2217/bmm-2020-0826. Epub 2021 Jul 22.
Obstructive sleep apnea syndrome (OSAS) is one of the most common sleep-related breathing disorders. The aim of this study was to improve diagnostics in OSAS using blood circulating biomarkers. We consider the potential of cardiac-specific miRNAs in the diagnosis and risk assessment of cardiovascular complications. Plasmatic levels of miR-1-3p, miR-133a-3p and miR-499a-5p were measured by reverse transcription-PCR and compared with the clinical status of OSAS patients and controls. The level of miR-499 was higher (p = 0.0343) in OSAS patients (mean expression: 0.00561) compared with the controls (mean expression: 0.00003), using the multivariate logistic regression. The role of miR-499 in gene expression regulation during hypoxia and our findings indicate that miR-499 could be a new diagnostic biomarker for OSAS.
阻塞性睡眠呼吸暂停综合征（OSAS）是最常见的与睡眠相关的呼吸障碍之一。本研究的目的是利用血液循环生物标志物改善OSAS的诊断。我们考虑了心脏特异性微小RNA在心血管并发症诊断和风险评估中的潜力。通过逆转录聚合酶链反应测定了miR-1-3p、miR-133a-3p和miR-499a-5p的血浆水平，并与OSAS患者和对照组的临床状况进行了比较。使用多变量逻辑回归分析，与对照组（平均表达量：0.00003）相比，OSAS患者（平均表达量：0.00561）中miR-499的水平更高（p = 0.0343）。miR-499在缺氧过程中基因表达调控中的作用以及我们的研究结果表明，miR-499可能是OSAS的一种新的诊断生物标志物。
