Rajput Dhavalsinh, Patel M P, Kumar Sushil, Patel Rumit, Katba Pankaj
Department of Genetics and Plant Breeding, BA College of Agriculture, Anand Agricultural University, Anand 388 110, India.
Department of Agricultural Biotechnology, Anand Agricultural University, Anand 388 110, India.
Heliyon. 2023 Mar 15;9(3):e14598. doi: 10.1016/j.heliyon.2023.e14598. eCollection 2023 Mar.
Castor ( L.) is an important industrial versatile non-edible oilseed C crop belongs to spurge family. Its oil has exceptional properties which provides an industrial importance to this crop. The present investigation was aimed to evaluate the genotypes of castor for Fusarium wilt reaction in pot followed by characterization of resistant genotypes for yield related traits in field and inter-genotype genetic diversity at DNA level. The percent disease incidence (PDI) among 50 genotypes ranged from 0 to 100%. A total of 36 genotypes were found wilt resistant (28 highly resistant and 8 resistant). ANOVA revealed that the genotypes MSS was significant for each trait studied, indicating the existence of plentiful variability in the experimental material. The morphological characterization showed that DCS-109 (73.30 cm) had a dwarf stature The genotype RG-1954 was superior for oil content (50.29%) with moderate for shelling out (67.63). RG-1673 was outstanding for seed boldness as 100 seed weight for this genotype was maximum (38.98 gm). JI-403 had maximum seed yield per plant (SYPP; 354.88 gm). SYPP positively associated with all traits except oil and seed length:breadth ratio. The path analysis revealed that the direct effects of NPR (0.549), TLFP (0.916), and CPP on SYPP are quite significant. A total of 38 alleles from 18 SSR markers were amplified in 36 genotypes. The NJ tree could divide 36 genotypes into three main clusters. AMOVA revealed 15% and 85% variance among and within subpopulations, respectively. Both morphological and SSR data demonstrated to be effective tools for discerning inter-genotypes diversity and categorizing high-yielding and disesae-tolerant castor genotypes lines.
蓖麻（Ricinus communis L.）是大戟科一种重要的工业用多用途非食用油籽作物。其油具有特殊性质，赋予了该作物重要的工业价值。本研究旨在评估蓖麻基因型在盆栽条件下对枯萎病的反应，随后在田间对抗病基因型的产量相关性状进行表征，并在DNA水平上分析基因型间的遗传多样性。50个基因型的发病率百分比（PDI）范围为0至100%。共发现36个基因型抗枯萎病（28个高抗和8个抗病）。方差分析表明，基因型MSS对所研究的每个性状都具有显著性，表明实验材料中存在丰富的变异性。形态学特征表明，DCS - 109（73.30厘米）植株矮小。基因型RG - 1954的含油率较高（50.29%），脱壳率适中（67.63）。RG - 1673的种子饱满度突出，该基因型的百粒重最大（38.98克）。JI - 403单株种子产量最高（SYPP；354.88克）。SYPP与除油和种子长宽比之外的所有性状呈正相关。通径分析表明，NPR（0.549）、TLFP（0.916）和CPP对SYPP的直接影响非常显著。从18个SSR标记中共扩增出38个等位基因，用于36个基因型分析。NJ树可将36个基因型分为三个主要类群。分子方差分析（AMOVA）表明，亚群体间和亚群体内的方差分别为15%和85%。形态学和SSR数据均证明是识别基因型间多样性以及对高产和抗病蓖麻基因型系进行分类的有效工具。
