Ahmad Zakia, Mumtaz Abdul Samad, Ghafoor Abdul, Ali Amjad, Nisar Mohammad
Department of Botany, University of Malakand, Chakdara Dir (Lower), Khyber Pakhtunkhawa, Pakistan,
Mol Biol Rep. 2014 Oct;41(10):6755-62. doi: 10.1007/s11033-014-3561-3. Epub 2014 Jul 14.
The exploration of genetically superior accessions is the key source of germplasm conservation and potential breeding material for the future. To meet the demand of better yielding chickpea cultivars in Pakistan the present study was organized to select more stable and resistant lines from indigenous as well as exotic chickpea germplasm obtained from Plant Genetic Resource Institute (PGRI), National Agricultural Research Centre, Islamabad, Pakistan. For the identification and evaluation of chickpea wilt resistant lines against Fusarium oxysporum f. sp. ciceris (Schlechtends), the germplasm was tested in the field for the selection of wilt resistant lines and the PCR based molecular markers were investigated to use Marker Assisted Selection (MAS) for selection of the desirable cultivars. In field trial, 70 % accessions were resistant to wilt disease, while the remaining 30 % have shown susceptibility to the disease. A total of 5 RAPD and 15 SSR markers were screened for molecular based characterization of wilt response. The data of molecular markers were scored by the presence (1) and absence (0) of allele and subjected to statistical analysis. The analysis was based on coefficient of molecular similarity using UPGMA and sorted the germplasm into two groups based on disease response. Among the total used RAPD/SSR primers, only TA194 SSR marker showed linkage to wilt resistant locus at 85 % probability. The linkage of a marker was reconfirmed by receiver operating characteristic curve. The use of the sorted wilt resistant genotypes through SSR marker TA194 can make available ample prospect in MAS breeding for yield improvement of the crop in Pakistan.
探索具有遗传优势的种质资源是未来种质资源保护和潜在育种材料的关键来源。为满足巴基斯坦对高产鹰嘴豆品种的需求，本研究旨在从巴基斯坦伊斯兰堡国家农业研究中心植物遗传资源研究所（PGRI）获取的本地及外来鹰嘴豆种质资源中筛选出更稳定、抗性更强的品系。为了鉴定和评估鹰嘴豆对尖镰孢菌鹰嘴豆专化型（Schlechtends）的抗枯萎病品系，在田间对种质资源进行了测试以筛选抗枯萎病品系，并研究了基于PCR的分子标记，以便利用标记辅助选择（MAS）来选择理想的品种。在田间试验中，70%的种质资源对枯萎病具有抗性，而其余30%表现出对该病的易感性。共筛选了5个RAPD标记和15个SSR标记用于基于分子的枯萎病抗性表征。分子标记数据根据等位基因的存在（1）和缺失（0）进行评分，并进行统计分析。分析基于使用UPGMA的分子相似系数，并根据病害反应将种质资源分为两组。在所有使用的RAPD/SSR引物中，只有TA194 SSR标记在85%的概率下与抗枯萎病基因座连锁。通过接收者操作特征曲线再次确认了一个标记的连锁关系。利用SSR标记TA194筛选出的抗枯萎病基因型在巴基斯坦作物产量改良的MAS育种中具有广阔前景。
