Department of Chemistry, Institute for Advanced Study, Division of Biomedical Engineering, Division of Life Science, State Key Laboratory of Molecular Neuroscience and Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology , Clear Water Bay, Kowloon, Hong Kong, China.
Anal Chem. 2014 Jan 21;86(2):1263-8. doi: 10.1021/ac403616c. Epub 2013 Dec 27.
Cardiolipin (CL) is a unique phospholipid found in mitochondrial inner membrane. It is a key component for mitochondrial function in both respiration and apoptosis. The level of CL is an important parameter for investigating these intracellular events and is a critical indicator of a number of diseases associated with mitochondrial respiratory functions. 10-Nonyl acridine orange (NAO) is the only fluorescent dye currently available for CL detection. However, the performance of NAO is far from satisfactory in terms of selectivity and sensitivity. In this work, we report an aggregation-induced emission-active fluorogen, TTAPE-Me, for CL detection and quantification. With improved sensitivity and excellent selectivity to CL over other major mitochondrial membrane lipids, TTAPE-Me could serve as a valuable fluorescent sensor for CL quantification. The use of TTAPE-Me for the quantification of isolated mitochondria is also demonstrated.
心磷脂（CL）是一种存在于线粒体内膜的独特磷脂。它是线粒体呼吸和凋亡功能的关键组成部分。CL 的水平是研究这些细胞内事件的一个重要参数，也是与线粒体呼吸功能相关的许多疾病的关键指标。10-壬基吖啶橙（NAO）是目前唯一可用于 CL 检测的荧光染料。然而，NAO 在选择性和灵敏度方面的性能远不能令人满意。在这项工作中，我们报告了一种用于 CL 检测和定量的聚集诱导发射活性荧光团 TTAPE-Me。TTAPE-Me 对 CL 的灵敏度和选择性均得到了提高，对其他主要的线粒体膜脂质具有优异的选择性，可作为 CL 定量的有价值的荧光传感器。还展示了 TTAPE-Me 用于分离线粒体定量的用途。
