Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Reduit 80837, Mauritius.
IOC Science and Communication Centre on Harmful Algae, Biological Institute, University of Copenhagen, Universitetsparken 4, DK-2100 Copenhagen, Denmark.
Mar Drugs. 2023 Mar 28;21(4):216. doi: 10.3390/md21040216.
Microalgae are unicellular organisms and commonly present in the euphotic zone of marine ecosystems. From the western coast of Mauritius, three strains of species were isolated from macrophytes and cultured under standard laboratory conditions. Morphologies were examined by light, fluorescence, and scanning electron microscopy, and phylogenetic analyses were based on partial large subunit LSU rDNA (D1-D2) and ITS1-5.8S-ITS2 (ITS) regions. Three species, including the complex, , and complex, were identified. The antimicrobial activities were assayed against potential human pathogenic bacterial strains. The highest zone of inhibition was recorded for intracellular and extracellular protein extracts of against . The polysaccharide extracts of the complex had a higher zone of inhibition (24 ± 0.4 mm) against MRSA at a minimum concentration of 0.625 μg/mL. The extracts from the three species had different levels of activity against the pathogens used, and this can be of scientific interest in the search for antibiotics from natural marine sources.
微藻是单细胞生物,通常存在于海洋生态系统的透光带中。从毛里求斯西海岸,从大型植物中分离出 3 株 种,并在标准实验室条件下培养。通过光学显微镜、荧光显微镜和扫描电子显微镜观察形态,并基于大亚基 LSU rDNA(D1-D2)和 ITS1-5.8S-ITS2(ITS)区域进行系统发育分析。鉴定出 3 种 种,包括 复合体、 和 复合体。对抗潜在的人类病原菌的抗菌活性进行了测定。对 的细胞内和细胞外蛋白提取物的抑制带最高。在最低浓度为 0.625 μg/mL 时, 复合体的多糖提取物对 MRSA 的抑制带(24 ± 0.4 mm)更高。来自这 3 种 种的提取物对所用病原体具有不同水平的活性,这在从天然海洋来源寻找抗生素方面具有科学意义。