Oyeku Oyeshina Gideon, Mandal Subir Kumar
Division of Applied Phycology and Biotechnology, CSIR-Central Salt & Marine Chemicals Research Institute, Gijubhai Badheka Marg, Bhavnagar, 364002, Gujarat, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India; Bowen University, P. M. B 284, Iwo, Osun State, Nigeria.
Division of Applied Phycology and Biotechnology, CSIR-Central Salt & Marine Chemicals Research Institute, Gijubhai Badheka Marg, Bhavnagar, 364002, Gujarat, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Toxicon. 2021 Jun;196:32-43. doi: 10.1016/j.toxicon.2021.03.017. Epub 2021 Mar 26.
Prorocentrum lima (CSIRCSMCRI005) was isolated from the coastal seawater of Thonithurai, Tamil Nadu, India. Morphology of the isolate was studied using light microscopy (LM) and scanning electron microscopy (SEM) while phylogenetic analyses of the internal transcribed spacer region (ITS1-5.8s-ITS2), 18S rDNA, and large subunit (LSU) rDNA were also carried out. Growth of the isolate was studied, and okadaic acid (OA) production was examined using liquid chromatography with electrospray ionization and quadrupole time of flight mass spectroscopy (LC-ESI-Q-ToF-MS). Morphological features observed including oval cell shape with a broad middle region, narrow anterior and round posterior end, large central pyrenoid with starch sheath, smooth thecal surface, and V-shaped periflagellar area consisting of eight platelets matched with the description of the type species and those reported elsewhere. The ITS, 18S, and LSU sequence phylogenetic analysis revealed that the isolate was closely related to other strains reported from the pacific. The growth rate (μ) was 0.05 div. day. P. lima CSIRCSMCRI005 produced okadaic acid and related esters. The production of free and total OA was 20.12 ± 4.77 and 22.30 fg cell respectively. The findings of this study contribute useful information concerning the regional risk of diarrheic shellfish poisoning in the North East Indian Ocean and the global distribution and toxic potential of Prorocentrum lima. Further studies on the ecophysiology of this strain will be helpful. This manuscript reports the detailed morphological, phylogenetic, and toxicological characterization of this species from the Bay of Bengal and the North Indian Ocean as a whole.
利玛原甲藻（CSIRCSMCRI005）分离自印度泰米尔纳德邦托尼图赖的沿海海水。使用光学显微镜（LM）和扫描电子显微镜（SEM）对分离株的形态进行了研究，同时还对内部转录间隔区（ITS1-5.8s-ITS2）、18S核糖体DNA（rDNA）和大亚基（LSU）rDNA进行了系统发育分析。研究了分离株的生长情况，并使用电喷雾电离和四极杆飞行时间质谱联用的液相色谱法（LC-ESI-Q-ToF-MS）检测了冈田酸（OA）的产生。观察到的形态特征包括椭圆形细胞，中部宽阔，前端狭窄，后端圆形，大型中央蛋白核有淀粉鞘，鞘表面光滑，由八个血小板组成的V形鞭毛周区，与模式种及其他地方报道的描述相符。ITS、18S和LSU序列的系统发育分析表明，该分离株与从太平洋报道的其他菌株密切相关。生长速率（μ）为0.05 次/天。利玛原甲藻CSIRCSMCRI005产生了冈田酸及相关酯类。游离OA和总OA的产量分别为每细胞20.12±4.77和22.30 fg。本研究结果为印度洋东北部腹泻性贝类中毒的区域风险以及利玛原甲藻的全球分布和毒性潜力提供了有用信息。对该菌株生态生理学的进一步研究将有所帮助。本手稿报告了来自孟加拉湾和整个北印度洋的该物种详细的形态、系统发育和毒理学特征。
