Park Soon Young, Lee Eun Hwa, Kim Dokyun, Song Young Goo, Jeong Su Jin
Division of Infectious Diseases, Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 06273, Republic of Korea.
Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
Antibiotics (Basel). 2023 Apr 13;12(4):748. doi: 10.3390/antibiotics12040748.
is the primary causative agent of gastritis, gastric ulcers, duodenal ulcers, gastric cancer, and peripheral B-cell lymphoma. eradication often fails due to elevated antibiotic resistance. However, no previous studies have thoroughly examined amoxicillin resistance. Here, the objective was to identify clinical strains of with amoxicillin resistance and to analyze single-nucleotide polymorphisms (SNPs) associated with amoxicillin resistance. From March 2015 to June 2019, genotypic and phenotypic amoxicillin resistance was analyzed using an E-test and whole-genome sequencing (WGS). Analysis of 368 clinical strains confirmed amoxicillin resistance in 31 strains (resistance rate of 8.7%). The genomes were extracted from nine resistant (<0.125 mg/L) strains, and WGS was performed for genetic analysis. WGS analysis identified SNPs present in , , , , , and in all nine isolates. Some of these genes may be related to amoxicillin resistance. A total of six SNPs (A69V, V374L, S414R, T503I, A592D, and R435Q) were identified in PBP2 of H-8, the most resistant strain. We predict that these six SNPs are associated with high amoxicillin resistance. Amoxicillin resistance should be considered in the clinical setting for the treatment failure of eradication.
是胃炎、胃溃疡、十二指肠溃疡、胃癌和外周B细胞淋巴瘤的主要病原体。由于抗生素耐药性升高，根除治疗常常失败。然而，以前没有研究彻底检查过阿莫西林耐药性。在此，目的是鉴定具有阿莫西林耐药性的临床菌株，并分析与阿莫西林耐药性相关的单核苷酸多态性（SNP）。从2015年3月至2019年6月，使用E-test和全基因组测序（WGS）分析基因型和表型阿莫西林耐药性。对368株临床菌株的分析证实31株存在阿莫西林耐药性（耐药率为8.7%）。从9株耐药（<0.125mg/L）菌株中提取基因组，并进行WGS以进行遗传分析。WGS分析在所有9株分离株中鉴定出存在于、、、、、和中的SNP。这些基因中的一些可能与阿莫西林耐药性有关。在耐药性最强的菌株H-8的PBP2中总共鉴定出6个SNP（A69V、V374L、S414R、T503I、A592D和R435Q）。我们预测这6个SNP与高阿莫西林耐药性相关。在根除治疗失败的临床情况下应考虑阿莫西林耐药性。
