Centro de Estudios Avanzados en Fruticultura (CEAF), Camino Las Parcelas 882, km 105 Ruta 5 Sur, Sector Los Choapinos, Rengo 2940000, Chile.
Genes (Basel). 2023 Apr 19;14(4):940. doi: 10.3390/genes14040940.
Aquaporins (AQPs) are integral transmembrane proteins well known as channels involved in the mobilization of water, small uncharged molecules and gases. In this work, the main objective was to carry out a comprehensive study of AQP encoding genes in (cv. Mazzard F12/1) on a genome-wide scale and describe their transcriptional behaviors in organs and in response to different abiotic stresses. A total of 28 non-redundant AQP genes were identified in spp. Genomes, which were phylogenetically grouped into five subfamilies (seven PIPs, eight NIPs, eight TIPs, three SIPs and two XIPs). Bioinformatic analyses revealed a high synteny and remarkable conservation of structural features among orthologs of different genomes. Several cis-acting regulatory elements (CREs) related to stress regulation were detected (ARE, WRE3, WUN, STRE, LTR, MBS, DRE, AT-rich and TC-rich). The above could be accounting for the expression variations associated with plant organs and, especially, each abiotic stress analyzed. Gene expressions of different PruavAQPs were shown to be preferentially associated with different stresses. PruavXIP2;1 and PruavXIP1;1 were up-regulated in roots at 6 h and 72 h of hypoxia, and in PruavXIP2;1 a slight induction of expression was also detected in leaves. Drought treatment strongly down-regulated PruavTIP4;1 but only in roots. Salt stress exhibited little or no variation in roots, except for PruavNIP4;1 and PruavNIP7;1, which showed remarkable gene repression and induction, respectively. Interestingly, PruavNIP4;1, the AQP most expressed in cherry roots subjected to cold temperatures, also showed this pattern in roots under high salinity. Similarly, PruavNIP4;2 consistently was up-regulated at 72 h of heat and drought treatments. From our evidence is possible to propose candidate genes for the development of molecular markers for selection processes in breeding programs for rootstocks and/or varieties of cherry.
水通道蛋白（AQP）是一种整合的跨膜蛋白，作为参与水、小非电荷分子和气体迁移的通道而广为人知。在这项工作中，主要目的是在全基因组范围内对 （cv. Mazzard F12/1）中的 AQP 编码基因进行全面研究，并描述它们在器官中的转录行为以及对不同非生物胁迫的反应。在 spp. 基因组中总共鉴定出 28 个非冗余的 AQP 基因，这些基因在系统发育上分为五个亚家族（7 个 PIPs、8 个 NIPs、8 个 TIPs、3 个 SIPs 和 2 个 XIPs）。生物信息学分析表明，不同 基因组的同源基因具有高度的同线性和结构特征的显著保守性。检测到与应激调节相关的多个顺式作用调节元件（CREs）（ARE、WRE3、WUN、STRE、LTR、MBS、DRE、富含 AT 和富含 TC）。这可以解释与植物器官相关的表达变化，特别是与每种分析的非生物胁迫相关的表达变化。不同 PruavAQP 的基因表达显示与不同胁迫的优先相关性。PruavXIP2;1 和 PruavXIP1;1 在缺氧 6 h 和 72 h 时在根中上调，在 PruavXIP2;1 中也检测到轻微的表达诱导。干旱处理强烈下调 PruavTIP4;1，但仅在根中。盐胁迫除了 PruavNIP4;1 和 PruavNIP7;1 外，在根中变化很小或没有，PruavNIP4;1 和 PruavNIP7;1 分别表现出显著的基因抑制和诱导。有趣的是，在冷胁迫下樱桃根中表达最丰富的 AQP PruavNIP4;1 在高盐度下也表现出这种模式。同样，PruavNIP4;2 在热和干旱处理 72 h 时始终上调。根据我们的证据，可以为砧木和/或樱桃品种的选择过程的分子标记的开发提出候选基因。
