Mirra Davida, Esposito Renata, Spaziano Giuseppe, Rafaniello Concetta, Iovino Pasquale, Cione Erika, Gallelli Luca, D'Agostino Bruno
Department of Environmental Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.
Campania Regional Centre for Pharmacovigilance and Pharmacoepidemiology, 80138 Naples, Italy.
J Clin Med. 2023 Apr 8;12(8):2775. doi: 10.3390/jcm12082775.
Atopy is an exaggerated IgE-mediated immune response to foreign antigens in which metabolic abnormalities of the leukotrienes (LTs) pathway play a crucial role. Recent studies have described sex as a key variable in LT biosynthesis, partly explaining why treatment with anti-LT drugs in atopic subjects leads to better control of symptoms in women. In addition, variability in LT production is often associated with single nucleotide polymorphisms (SNPs) in the arachidonate 5-lipoxygenase () gene, which encodes the leukotriene-synthesizing enzyme machinery, 5-lipoxygenase (). This study aimed to investigate whether two SNPs of are implicated in sex differences in allergic diseases in a prospective cohort of 150 age- and sex-matched atopic and healthy subjects. Rs2029253 and rs2115819 were genotyped using allele-specific RT-PCR, and serum levels of and were measured by ELISA. Both polymorphisms are significantly more common in women than in men, and their influences on LT production vary as a function of sex, leading to a decrease in men's and an increase in women's serum levels of and . These data represent a new resource for understanding sex-related differences in lung inflammatory diseases, partly explaining why women are more likely to develop allergic disorders than men.
特应性是一种针对外来抗原的、由IgE介导的过度免疫反应，其中白三烯（LTs）途径的代谢异常起着关键作用。最近的研究表明，性别是LT生物合成中的一个关键变量，这在一定程度上解释了为什么在特应性受试者中使用抗LT药物治疗能更好地控制女性的症状。此外，LT产生的变异性通常与花生四烯酸5-脂氧合酶（）基因中的单核苷酸多态性（SNP）有关，该基因编码白三烯合成酶机制——5-脂氧合酶（）。本研究旨在调查在一个由150名年龄和性别匹配的特应性和健康受试者组成的前瞻性队列中，的两个SNP是否与过敏性疾病中的性别差异有关。使用等位基因特异性RT-PCR对rs2029253和rs2115819进行基因分型，并通过ELISA测量血清中和的水平。这两种多态性在女性中比在男性中显著更常见，并且它们对LT产生的影响随性别而变化，导致男性血清中和水平降低，女性血清中和水平升高。这些数据为理解肺部炎症性疾病中的性别相关差异提供了新的资源，部分解释了为什么女性比男性更容易患过敏性疾病。
