de Souza Thalisson A, Rodrigues Gabriela C S, de Souza Pedro H N, Abreu Lucas S, Pereira Laiane C O, da Silva Marcelo S, Tavares Josean F, Scotti Luciana, Scotti Marcus Tullius
Multi-User Laboratory for Characterization and Analysis, Program of Natural and Synthetic Bioactive Products (PgPNSB), Health Sciences Center, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil.
Laboratory of Cheminformatics, Program of Natural and Synthetic Bioactive Products (PgPNSB), Health Sciences Center, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil.
Life (Basel). 2023 Apr 17;13(4):1034. doi: 10.3390/life13041034.
The use of agrochemicals has become a standard practice worldwide to ensure the productivity and quality of sugarcane crops. This study aimed to analyze the metabolic changes in sugarcane culms treated with five different nematicides. The experimental design was randomized in blocks, and agro-industrial and biometric variables were evaluated. The samples were extracted and then analyzed using LC-MS, LC-MS/MS, and LC-HRMS. The data obtained were submitted to statistical methods (PCA and PLS). Fragmentation patterns, retention time, and UV absorptions of the main features were analyzed. The plantations treated with carbosulfan (T4) obtained higher agricultural productivity and total recoverable sugar (TRS), while the use of benfuracarb (T3) was associated with lower growth and lower TRS. Statistical analysis revealed the contribution of the features at / 353 and / 515, assigned as chlorogenic acids, which discriminated the groups. The MS profile also supported the occurrence of flavonoids (C-glycosides and O-glycosides) in the samples.
为确保甘蔗作物的产量和质量，使用农用化学品已成为全球的标准做法。本研究旨在分析用五种不同杀线虫剂处理的甘蔗茎杆的代谢变化。实验设计采用随机区组设计，并对农艺工业和生物特征变量进行评估。提取样品后，使用液相色谱-质谱联用仪（LC-MS）、液相色谱-串联质谱联用仪（LC-MS/MS）和液相色谱-高分辨质谱联用仪（LC-HRMS）进行分析。将获得的数据提交给统计方法（主成分分析（PCA）和偏最小二乘法（PLS））。分析了主要特征的碎片模式、保留时间和紫外吸收。用丁硫克百威处理的种植园（T4）获得了更高的农业生产力和总可回收糖（TRS），而使用丙硫克百威（T3）则与较低的生长和较低的TRS相关。统计分析揭示了在m/z 353和m/z 515处被鉴定为绿原酸的特征的贡献，这些特征区分了不同的组。质谱图也支持样品中黄酮类化合物（碳苷和氧苷）的存在。
