Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, Universidade Federal da Paraíba, João Pessoa, Brazil.
Faculdade de Farmácia, Universidade Federal da Bahia, Salvador, Brazil.
PLoS One. 2019 Oct 29;14(10):e0224575. doi: 10.1371/journal.pone.0224575. eCollection 2019.
Limonoids, quinolone alkaloids and chromones have been reported as constituents of Dictyoloma vandellianum Adr. Juss. (Rutaceae). Although those compounds are known for their biological activities, only the anti-inflammatory activity of chromones isolated from the underground parts has been evaluated. There are no studies of the pharmacological properties of the aerial parts of D. vandellianum. The present study was carried out to determine the phytochemical profile and antinociceptive activity of the methanol extract, fractions and isolated compounds of leaves of D. vandellianum. The phytochemical profile was performed by HLPC-DAD-ESIMSn and pure substances obtained were characterized by MS and NMR spectroscopy. The antinociceptive activity was assessed using the formalin assay in mice, and the motor function in the rotarod test. ME and all the fractions obtained from ME produced antinociceptive effects. Among them, the ethyl ether fraction was the most active. Data from HPLC-DAD-ESIMSn showed that the ethyl ether fraction presented 42 compounds. The major compounds isolated from this fraction-gallic acid, methyl gallate and 1,2,6-tri-O-galloyl-β-d-glucopyranose-were tested and produced antinociceptive effects. Gallic acid, methyl gallate and 1,2,6-tri-O-galloyl-β-d-glucopyranose at antinociceptive doses did not affect the motor performance in mice in the rotarod test. This work is the first report of the occurrence of gallotanins in D. vandellianum. In addition, the pharmacological study showed that D. vandellianum leaves present antinociceptive activity, probably induced by gallic acid, methyl gallate and 1,2,6-tri-O-galloyl-β-d-glucopyranose.
柠檬苦素、喹诺酮生物碱和色酮已被报道为 Dictyoloma vandellianum Adr. Juss.（芸香科）的成分。尽管这些化合物以其生物活性而闻名，但仅对从地下部分分离出的色酮的抗炎活性进行了评估。目前还没有关于 D. vandellianum 地上部分的药理特性的研究。本研究旨在确定 D. vandellianum 叶片甲醇提取物、馏分和分离化合物的植物化学特征和镇痛活性。植物化学特征通过 HLPC-DAD-ESIMSn 进行，获得的纯物质通过 MS 和 NMR 光谱进行表征。镇痛活性通过在小鼠中使用福氏试剂测定法和旋转棒试验中的运动功能进行评估。ME 和从 ME 获得的所有馏分均产生镇痛作用。其中，乙基醚馏分最为活跃。HPLC-DAD-ESIMSn 的数据显示，乙基醚馏分中存在 42 种化合物。从该馏分中分离出的主要化合物-没食子酸、甲基没食子酸和 1,2,6-三-O-没食子酰基-β-d-吡喃葡萄糖-也进行了测试，并产生了镇痛作用。在镇痛剂量下，没食子酸、甲基没食子酸和 1,2,6-三-O-没食子酰基-β-d-吡喃葡萄糖对旋转棒试验中的小鼠运动性能没有影响。这是首次报道 D. vandellianum 中存在没食子单宁。此外，药理研究表明，D. vandellianum 叶片具有镇痛活性，可能是由没食子酸、甲基没食子酸和 1,2,6-三-O-没食子酰基-β-d-吡喃葡萄糖诱导的。
