Yousuf Sufiara, Jamal Mamdoh T, Al-Farawati Radwan Kahalid, Al-Mur Bandar Ahmad, Singh Rahul
Department of Zoology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara 144411, Punjab, India.
Department of Marine Biology, Faculty of Marine Sciences, King Abdul-Aziz University, Jeddah 21589, Saudi Arabia.
Microorganisms. 2023 Mar 25;11(4):842. doi: 10.3390/microorganisms11040842.
Probiotics play vital roles in improving growth, survival, and immune responses and inhibit the growth of pathogenic bacteria in freshwater fish. This study was conducted to isolate potential probiotics from and and to evaluate their effect on fingerlings. Among the isolates, PBG9D and BCS10 (1) exhibited antimicrobial activity against the fish pathogen . Both strains showed tolerance to acidic and alkaline pH (2, 3, 4, 7, and 9) and bile salts (0.3%) and exhibited strong adhesion capacity. After in-vitro assessment, these strains were evaluated on the growth performances of rohu fingerlings challenged by for 4 weeks. The study consisted of six groups, each containing 6 fish. Group (I) was the control, fed a basal diet; group (II) contained a pathogen and was also fed a basal diet; group (III & IV) was given a probiotic supplemented experimental diet; Fourth group (V & VI) contained a pathogen and was given a probiotic supplemented experimental diet. After the 12th day of experiment, rohu fingerlings of pathogen (II) and probiotic + pathogen (V & VI) groups were intraperitoneally injected with 0.1 mL of . After 4 weeks, no significant differences in weight gain, weight gain %, and feed conversion ratio were observed in probiotic (III & IV)- fed groups compared to control. However, the specific growth rate was significantly improved in probiotic fed groups compared to other groups. Survival rate and condition factor were significantly similar in all groups. After injection, abnormal swimming, loss of appetite and weight loss were observed in the pathogen (II) group, while no such symptoms were found in the probiotic + pathogen (V & VI)- groups, confirming the effects of probiotics. The overall results of the study revealed that dietary supplementation with strains could improve the specific growth rate and disease resistance against in .
益生菌在促进淡水鱼生长、提高存活率和免疫反应以及抑制病原菌生长方面发挥着重要作用。本研究旨在从[具体来源1]和[具体来源2]中分离潜在的益生菌，并评估它们对[鱼的品种]鱼种的影响。在分离出的菌株中，PBG9D和BCS10（1）对鱼类病原菌[病原菌名称]表现出抗菌活性。这两种菌株都对酸性和碱性pH值（2、3、4、7和9）以及胆盐（0.3%）具有耐受性，并表现出很强的黏附能力。经过体外评估后，对这两种菌株在受到[病原菌名称]攻击4周的[鱼的品种]鱼种生长性能方面进行了评估。该研究包括六组，每组有6条鱼。第一组（I）为对照组，投喂基础饲料；第二组（II）含有病原菌，也投喂基础饲料；第三组和第四组（III & IV）给予添加益生菌的实验饲料；第五组和第六组（V & VI）含有病原菌，并给予添加益生菌的实验饲料。实验第12天后，对病原菌组（II）以及益生菌 + 病原菌组（V & VI）的[鱼的品种]鱼种腹腔注射0.1 mL的[病原菌名称]。4周后，与对照组相比，投喂益生菌的第三组和第四组（III & IV）在体重增加、体重增加百分比和饲料转化率方面没有显著差异。然而，与其他组相比，投喂益生菌的组的特定生长率显著提高。所有组的存活率和肥满度显著相似。注射后，病原菌组（II）出现异常游泳、食欲不振和体重减轻的情况，而在益生菌 + 病原菌组（V & VI）中未发现此类症状，证实了益生菌的作用。该研究的总体结果表明，在饲料中添加[菌株名称]菌株可以提高[鱼的品种]对[病原菌名称]的特定生长率和抗病能力。
