Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China.
Molecules. 2023 Apr 10;28(8):3346. doi: 10.3390/molecules28083346.
A derivatization method combined with high-performance liquid chromatography-fluorescence detection (HPLC-FLD) was used to evaluate the dissipation, residue distribution and risk assessment of emamectin benzoate in whole longan and pulp. The average recoveries were 82-111% with relative standard deviation (RSD) less than 11%. The limit of quantification (LOQ) was 0.001 mg/kg in longan and pulp. The half-lives were 3.3-4.2 days. The terminal residues in whole longan were <0.001-0.025 mg/kg applied two and three times at two levels of dosage with PHIs of 10, 14, and 21 days. The residues in whole longan had a higher quantity than those in the pulp, and the terminal residues of pulp were all lower than LOQ (0.001 mg/kg). The chronic risk of emamectin benzoate was not negligible to humans depending on ADI% value, which was higher than 1; and the acute risk was acceptable to the consumer. This study could provide guidance for the safe use of emamectin benzoate in longan and serve as a reference for the establishment of maximum residue limits (MRLs) in China.
采用衍生化结合高效液相色谱-荧光检测法（HPLC-FLD）评价了在整个桂圆和果肉中苯甲酰丁醚脲的消解、残留分布和风险评估。平均回收率为 82-111%，相对标准偏差（RSD）小于 11%。在桂圆和果肉中的定量限（LOQ）为 0.001mg/kg。半衰期为 3.3-4.2 天。在两次施药，两个剂量水平下，施药 PHI 为 10、14 和 21 天时，在整个桂圆中的最终残留量<0.001-0.025mg/kg。整个桂圆中的残留量高于果肉中的残留量，果肉中的最终残留量均低于 LOQ（0.001mg/kg）。根据 ADI%值，苯甲酰丁醚脲对人类的慢性风险不可忽视，高于 1；急性风险对消费者是可以接受的。本研究可为苯甲酰丁醚脲在桂圆上的安全使用提供指导，为中国制定最大残留限量（MRLs）提供参考。
