Inokuchi Y, Takahashi R, Hirose T, Inayama S, Jacobson A B, Hirashima A
J Biochem. 1986 Apr;99(4):1169-80. doi: 10.1093/oxfordjournals.jbchem.a135580.
The complete nucleotide sequence of the RNA coliphage GA, a group II phage, is presented. The entire genome comprises 3466 bases. Three large open reading frames were identified, which correspond to the maturation protein gene (390 amino acids), the coat protein gene (129 amino acids) and the replicase beta-subunit protein gene (531 amino acids). In addition, untranslated regions occur at the 5' (135 bases) and 3' (122 bases) ends of the molecule. Two intercistronic untranslated regions occur between the cistrons for the maturation and coat proteins, and between the coat and beta-subunit proteins. We have compared the nucleotide sequence of GA RNA with the published sequence of MS2 RNA, and show that they are related. The comparative structures of two important regulatory regions are presented; the coat protein binding site which is involved in translational repression of the replicase beta-subunit protein gene, and a hairpin in a region proximal to the lysis protein gene.
本文给出了RNA噬菌体GA（一种II组噬菌体）的完整核苷酸序列。其整个基因组由3466个碱基组成。鉴定出了三个大的开放阅读框，分别对应成熟蛋白基因（390个氨基酸）、外壳蛋白基因（129个氨基酸）和复制酶β亚基蛋白基因（531个氨基酸）。此外，在分子的5'端（135个碱基）和3'端（122个碱基）存在非翻译区。在成熟蛋白和顺反子与外壳蛋白之间，以及外壳蛋白和顺反子与β亚基蛋白之间存在两个顺反子间非翻译区。我们将GA RNA的核苷酸序列与已发表的MS2 RNA序列进行了比较，结果表明它们具有相关性。文中给出了两个重要调控区的比较结构；一个是参与复制酶β亚基蛋白基因翻译抑制的外壳蛋白结合位点，另一个是裂解蛋白基因近端区域的发夹结构。
