IV 组 RNA 大肠杆菌噬菌体 SP 的全核苷酸序列分析。
Analysis of the complete nucleotide sequence of the group IV RNA coliphage SP.
作者信息
Inokuchi Y, Jacobson A B, Hirose T, Inayama S, Hirashima A
机构信息
Department of Molecular Biology, School of Medicine, Keio University, Tokyo, Japan.
出版信息
Nucleic Acids Res. 1988 Jul 11;16(13):6205-21. doi: 10.1093/nar/16.13.6205.
We report the nucleotide sequence of the Group IV RNA bacteriophage SP. The entire sequence is 4276 nucleotides long. Four cistrons have been identified by comparison with the related Group III phage Q beta. The maturation protein contains 449 amino acids, the coat protein contains 131 amino acids, the read-through protein contains 330 amino acids and the replicase beta-subunit contains 575 amino acids. SP is 59 nucleotides longer than Q beta. We have analyzed both sequence and structural conservation between SP and Q beta and shown that the sequences for the coat and central region of the replicase are strongly conserved between the two genomes. We also show that the S and M replicase binding sites of Q beta are strongly conserved in SP. Interestingly, the base composition of SP and Q beta differ significantly from one another, and most of the differences can be accounted for by a strong preponderance of U in the third position of each codon of Q beta relative to SP. We also compare conserved hairpins associated with potential coat protein and replicase binding sites.
我们报道了IV组RNA噬菌体SP的核苷酸序列。整个序列长4276个核苷酸。通过与相关的III组噬菌体Qβ比较，已鉴定出四个顺反子。成熟蛋白含有449个氨基酸，外壳蛋白含有131个氨基酸，通读蛋白含有330个氨基酸，复制酶β亚基含有575个氨基酸。SP比Qβ长59个核苷酸。我们分析了SP和Qβ之间的序列和结构保守性，结果表明，两个基因组之间外壳蛋白和复制酶中心区域的序列高度保守。我们还表明，Qβ的S和M复制酶结合位点在SP中也高度保守。有趣的是，SP和Qβ的碱基组成彼此有显著差异，并且大多数差异可以归因于相对于SP，Qβ每个密码子第三位的U占优势。我们还比较了与潜在外壳蛋白和复制酶结合位点相关的保守发夹结构。
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