乙型肝炎表面抗原主要多肽中天冬酰胺连接糖链的结构
Structure of asparagine-linked sugar chains in the major polypeptide of hepatitis B surface antigen.
作者信息
Hanaoka K, Takasaki S, Kobata A, Miyamoto H, Nakamura T, Mayumi M
出版信息
J Biochem. 1986 Apr;99(4):1273-6. doi: 10.1093/oxfordjournals.jbchem.a135591.
Asparagine-linked sugar chains were quantitatively released from hepatitis B surface antigen on hydrazinolysis followed by N-acetylation and NaB3H4 reduction. The released oligosaccharides were composed of two major sialylated components and a trace of a neutral component. From the results of the combination of sequential exoglycosidase digestion and methylation analysis, the structures of the acidic and the neutral components were deduced to be NeuAc alpha 2----6Gal beta 1----4GlcNAc beta 1----2Man alpha 1----3(+/- NeuAc alpha 2----6Gal beta 1----4GlcNAc beta 1----2Man alpha 1----6)Man beta 1----4GlcNAc beta 1----4GlcNAcOT and Gal beta 1----4GlcNAc beta 1----2Man alpha 1----3(Gal beta 1----4GlcNAc beta 1----2Man alpha 1----6)Man beta 1----4GlcNAc beta 1----4GlcNAcOT, respectively.
在肼解后，通过N - 乙酰化和NaB3H4还原从乙型肝炎表面抗原中定量释放出天冬酰胺连接的糖链。释放出的寡糖由两种主要的唾液酸化成分和微量的中性成分组成。根据顺序外切糖苷酶消化和甲基化分析相结合的结果，推断酸性成分和中性成分的结构分别为NeuAcα2----6Galβ1----4GlcNAcβ1----2Manα1----3(+/- NeuAcα2----6Galβ1----4GlcNAcβ1----2Manα1----6)Manβ1----4GlcNAcβ1----4GlcNAcOT和Galβ1----4GlcNAcβ1----2Manα1----3(Galβ1----4GlcNAcβ1----2Manα1----6)Manβ1----4GlcNAcβ1----4GlcNAcOT。
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