冷不溶性球蛋白的糖链。一种与纤连蛋白相关的蛋白质。

The sugar chains of cold-insoluble globulin. A protein related to fibronectin.

作者信息

Takasaki S, Yamashita K, Suzuki K, Iwanaga S, Kobata A

出版信息

J Biol Chem. 1979 Sep 10;254(17):8548-53.

Abstract

Cold-insoluble globulin isolated from bovine plasma contains six asparagine-linked sugar chains in 1 molecule (a dimeric form). These sugar chains were released from the polypeptide backbone by hydrazinolysis and labeled by reduction with NaB[3H]4. Most of these sugar chains contain N-acetylneuraminic acid and can be separated by paper electrophoresis. By combination of sequential exoglycosidase digestion and methylation study, their structures were elucidated as Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc, NeuAc alpha 2 leads to 6 or 4Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(NeuAc alpha 2 leads to 4 or 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc, NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6[NeuAc alpha 2 4Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 3]-Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc and NeuAc alpha 2 leads to 4Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 6[NeuAc alpha 2 leads to 4Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 3]man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc.

摘要

从牛血浆中分离出的冷不溶性球蛋白在1个分子（二聚体形式）中含有6条天冬酰胺连接的糖链。这些糖链通过肼解从多肽主链上释放出来，并用NaB[3H]4还原进行标记。这些糖链大多含有N-乙酰神经氨酸，可通过纸电泳分离。通过顺序外切糖苷酶消化和甲基化研究相结合，阐明了它们的结构为Galβ1→4GlcNAcβ1→2Manα1→6(NeuAcα2→6Galβ1→4GlcNAcβ1→2Manα1→3)Manβ1→4GlcNAcβ1→4GlcNAc、NeuAcα2→6或4Galβ1→4GlcNAcβ1→2Manα1→6(NeuAcα2→4或6Galβ1→4GlcNAcβ1→2Manα1→3)Manβ1→4GlcNAcβ1→4GlcNAc、NeuAcα2→6Galβ1→4GlcNAcβ1→2Manα1→6[NeuAcα2 4Galβ1→3(NeuAcα2→6)GlcNAcβ1→2Manα1→3]-Manβ1→4GlcNAcβ1→4GlcNAc和NeuAcα2→4Galβ1→3(NeuAcα2→6)GlcNAcβ1→2Manα1→6[NeuAcα2→4Galβ1→3(NeuAcα2→6)GlcNAcβ1→2Manα1→3]manβ1→4GlcNAcβ1→4GlcNAc。