Department of Food Science and Technology, Sunchon National University, Suncheon 57922, Republic of Korea.
Research Institute of Food Industry, Sunchon National University, Suncheon 57922, Republic of Korea.
Molecules. 2023 Apr 13;28(8):3417. doi: 10.3390/molecules28083417.
This study aimed to isolate and identify antibacterial compounds from () that are effective against the Streptococcus mutans KCCM 40105 strain. First, was extracted using varying concentrations of ethanol, and the resulting antibacterial activity was evaluated. The 30% ethanol extract of showed high activity. The fractionation and antibacterial activity of a 30% ethanol extract from were examined using five different solvents. Upon investigation of the antibacterial activity of the solvent fraction, the water and butanol fractions showed high activity, and no significant difference was found. Therefore, the butanol fraction was chosen for material exploration using silica gel column chromatography. A total of 24 fractions were obtained from the butanol portion using silica gel chromatography. The fraction with the highest antibacterial activity was Fr 7. From Fr 7, thirty-three sub-fractions were isolated, and sub-fraction 17 showed the highest level of antibacterial activity. A total of five peaks were obtained through the pure separation of sub-fraction 17 using HPLC. Peak 2 was identified as a substance exhibiting a high level of antibacterial activity. Based on the results of UV spectrometry, 13C-NMR, 1H-NMR, LC-MS, and HPLC analyses, the compound corresponding to peak number 2 was identified as tartaric acid.
本研究旨在从 （）中分离并鉴定出对变异链球菌 KCCM 40105 株有效的抗菌化合物。首先，用不同浓度的乙醇提取 ，并评估其抗菌活性。结果表明， 的 30%乙醇提取物具有较高的活性。采用五种不同溶剂对 30%乙醇提取物进行了分级和抗菌活性研究。在考察溶剂馏分的抗菌活性时，水和正丁醇馏分表现出较高的活性，且无显著差异。因此，选择正丁醇馏分进行硅胶柱层析的材料探索。通过硅胶柱层析，从正丁醇部分共得到 24 个馏分。具有最高抗菌活性的馏分为 Fr 7。从 Fr 7 中分离出 33 个亚馏分，其中亚馏分 17 显示出最高的抗菌活性。通过 HPLC 对亚馏分 17 进行纯分离，共得到 5 个峰。峰 2 被鉴定为具有高抗菌活性的物质。根据紫外光谱、13C-NMR、1H-NMR、LC-MS 和 HPLC 分析的结果，峰号 2 对应的化合物被鉴定为酒石酸。
