Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Medicine, Wuhan University of Science and Technology, Wuhan 430065, China.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Molecules. 2023 Apr 17;28(8):3542. doi: 10.3390/molecules28083542.
Applications of submicron-scale particles are of rising interest in separation science due to their favorable surface-to-volume ratio and their fabrication of highly ordered structures. The uniformly dense packing beds in columns assembled from nanoparticles combined with an electroosmotic flow-driven system has great potential in a highly efficient separation system. Here, we packed capillary columns using a gravity method with synthesized nanoscale C18-SiO particles having diameters of 300-900 nm. The separation of small molecules and proteins was evaluated in the packed columns on a pressurized capillary electrochromatography platform. The run-to-run reproducibility regarding retention time and peak area for the PAHs using a column packed with 300 nm C18-SiO particles were less than 1.61% and 3.17%, respectively. Our study exhibited a systematic separation analysis of small molecules and proteins based on the columns packed with submicron particles combined with the pressurized capillary electrochromatography (pCEC) platform. This study may provide a promising analytical approach with extraordinary column efficiency, resolution, and speed for the separation of complex samples.
由于具有较大的比表面积和易于制备高度有序结构的特点，亚微米级颗粒在分离科学中的应用越来越受到关注。由纳米颗粒组装而成的柱中均匀致密的填充床，结合电渗流驱动系统，在高效分离系统中具有很大的应用潜力。在这里，我们使用重力法在毛细管柱中填充了直径为 300-900nm 的合成纳米 C18-SiO 颗粒。在加压毛细管电色谱平台上，评估了填充柱对小分子和蛋白质的分离效果。使用填充有 300nm C18-SiO 颗粒的色谱柱进行 PAHs 分析时，保留时间和峰面积的运行间重现性分别小于 1.61%和 3.17%。本研究基于亚微米颗粒填充柱和加压毛细管电色谱（pCEC）平台，对小分子和蛋白质进行了系统的分离分析。该研究可能为分离复杂样品提供了一种具有非凡柱效、分辨率和速度的有前途的分析方法。
