Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
Talanta. 2022 Apr 1;240:123160. doi: 10.1016/j.talanta.2021.123160. Epub 2021 Dec 22.
The use of magnetic nanoparticles shows a steadily increasing technical importance. Particularly in medical technology disciplines such as cancer treatment, the potential of these special particles is increasing rapidly. Magnetic nanoparticles are particles with a submicron size, and consist mostly of magnetite-containing composites. An important quality parameter of such particles is a particle size distribution as narrow as possible, which can only be obtained to a certain degree by synthesis. Apart from ultracentrifugation, there are so far only methods on an analytical scale to narrow the size distribution as a post-processing step. We present a method based on magnetic chromatography, by which high separation efficiencies at yields of up to 99.9% are achieved. The novel technique is based on a competition between the magnetic interaction of the nanoparticles and the separation matrix, as well as the hydrodynamic forces. Furthermore, the method is extended using a continuous mode, namely simulated moving bed chromatography, to obtain potent space-time yields of up to 2.94 g/(L*h). For those reasons, this novel continuous magnetic chromatography method offers high potential for large-scale refinement of magnetic nanoparticles while fulfilling sophisticated quality criteria for high-technology applications.
磁性纳米粒子的应用显示出技术重要性的稳步增长。特别是在癌症治疗等医学技术领域，这些特殊粒子的潜力正在迅速增加。磁性纳米粒子是亚微米大小的粒子，主要由含有磁铁矿的复合材料组成。这些粒子的一个重要质量参数是尽可能窄的粒径分布，这只能通过合成在一定程度上获得。除了超速离心法外，迄今为止，只有在分析规模上的方法可以作为后处理步骤来缩小粒径分布。我们提出了一种基于磁色谱法的方法，通过该方法可以在高达 99.9%的收率下实现高分离效率。该新技术基于纳米粒子的磁相互作用与分离基质以及流体动力之间的竞争。此外，该方法通过连续模式（即模拟移动床色谱法）进行扩展，以获得高达 2.94 g/（L*h）的有效时空产率。由于这些原因，这种新型连续磁色谱法在满足高科技应用的复杂质量标准的同时，为大规模改进磁性纳米粒子提供了巨大潜力。
