Perles Lívia, Barreto Wanessa Teixeira Gomes, Santos Filipe Martins, Duarte Leidiane Lima, de Macedo Gabriel Carvalho, Barros-Battesti Darci Moraes, Herrera Heitor Miraglia, Machado Rosangela Zacarias, André Marcos Rogério
Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural, Jaboticabal 14884-900, Brazil.
Post-Graduation of Ecology and Conservation, Mato Grosso do Sul Federal University, Campo Grande 13471-410, Brazil.
Pathogens. 2023 Mar 30;12(4):538. doi: 10.3390/pathogens12040538.
Even though previous works showed molecular evidence of hemotropic spp. (hemoplasmas) in ring-tailed coatis () from Brazil, sp. has not been reported in these mammals so far. The present study aimed to detect the above-mentioned agents in coatis' blood and associated ectoparasites, assessing the association between these infections and red blood parameters. Between March 2018 and January 2019, coati (n = 97) blood samples, sp. ticks (2242 individual ticks, resulting in 265 pools), and louse (n = 59) were collected in forested urban areas from midwestern Brazil. DNA extracted from coatis' blood, and ectoparasite samples were submitted to quantitative PCR (qPCR) (16S rRNA) and conventional PCR (cPCR) (16S rRNA and 23S rRNA) for hemoplasmas and qPCR ( gene) and culturing (only blood) for spp. Two different hemoplasma genotypes were detected in blood samples: 71% coatis positive for myc1 and 17% positive for myc2. While 10% of ticks were positive for hemoplasmas (myc1), no louse was positive. The estimated bacterial load of hemoplasmas showed no association with anemia indicators. All coatis were negative for sp. in qPCR assay and culturing, albeit two sp. larvae pools, and 2 nymph pools were positive in the qPCR. The present work showed a high occurrence of hemoplasmas, with two distinct hemoplasma genotypes, in coatis from forested urban areas in midwestern Brazil.
尽管先前的研究表明，在来自巴西的环尾浣熊（）中存在嗜血性物种（血支原体）的分子证据，但迄今为止，尚未在这些哺乳动物中报道过 物种。本研究旨在检测浣熊血液和相关体外寄生虫中的上述病原体，评估这些感染与红细胞参数之间的关联。在2018年3月至2019年1月期间，从巴西中西部的城市森林地区采集了浣熊（n = 97）的血液样本、 属蜱虫（2242只个体蜱虫，共265组）和 属虱子（n = 59）。从浣熊血液和体外寄生虫样本中提取的DNA，分别进行血支原体的定量PCR（qPCR）（16S rRNA）和常规PCR（cPCR）（16S rRNA和23S rRNA）检测，以及 物种的qPCR（ 基因）检测和培养（仅针对血液）。在血液样本中检测到两种不同的血支原体基因型：71%的浣熊myc1呈阳性，17%的浣熊myc2呈阳性。虽然10%的蜱虫血支原体（myc1）呈阳性，但没有虱子呈阳性。血支原体的估计细菌载量与贫血指标之间没有关联。在qPCR检测和培养中，所有浣熊的 物种均为阴性，尽管有两组 物种幼虫样本和两组若虫样本在qPCR中呈阳性。本研究表明，在巴西中西部城市森林地区的浣熊中，血支原体的感染率很高，且存在两种不同的血支原体基因型。
