巴西潘塔纳尔湿地热带鸟类中新型蜱传无形体科基因型

Novel Tick-Borne Anaplasmataceae Genotypes in Tropical Birds from the Brazilian Pantanal Wetland.

作者信息

Alabí Córdova Amir Salvador, Fecchio Alan, Calchi Ana Cláudia, Dias Clara Morato, Mongruel Anna Claudia Baumel, das Neves Lorena Freitas, Lee Daniel Antonio Braga, Machado Rosangela Zacarias, André Marcos Rogério

机构信息

Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, Sao Paulo State University "Júlio de Mesquita Filho" (FCAV/UNESP), Jaboticabal 14884-900, Brazil.

Department of Ornithology, Academy of Natural Sciences of Drexel University, Philadelphia, PA 19103, USA.

出版信息

Microorganisms. 2024 May 10;12(5):962. doi: 10.3390/microorganisms12050962.

Despite numerous reports of Anaplasmataceae agents in mammals worldwide, few studies have investigated their occurrence in birds. The present study aimed to investigate the occurrence and molecular identity of Anaplasmataceae agents in birds from the Pantanal wetland, Brazil. Blood samples were collected from 93 different species. After DNA extraction, samples positive for the avian β-actin gene were subjected to both a multiplex quantitative real-time (q)PCR for and targeting the gene and to a conventional PCR for Anaplasmataceae agents targeting the 16S rRNA gene. As a result, 37 (7.4%) birds were positive for spp. and 4 (0.8%) for spp. in the qPCR assay; additionally, 13 (2.6%) were positive for Anaplasmataceae agents in the PCR targeting the 16S rRNA gene. The 16S rRNA sequences detected in , , and were positioned closely to sp. Magellanica. sequences detected in and grouped with . The 16S rRNA genotypes detected in and grouped with Allocryptoplasma. The 23S-5S genotypes detected in , , and were related to . In conclusion, novel genotypes of , , and Allocryptoplasma were detected in birds from the Pantanal wetland.

尽管全球有大量关于无形体科病原体在哺乳动物中出现的报道，但很少有研究调查其在鸟类中的存在情况。本研究旨在调查巴西潘塔纳尔湿地鸟类中无形体科病原体的存在情况及其分子特征。从93个不同物种采集了血样。DNA提取后，对禽β-肌动蛋白基因呈阳性的样本进行针对和靶向基因的多重定量实时（q）PCR，以及针对无形体科病原体靶向16S rRNA基因的常规PCR。结果，在qPCR检测中，37只（7.4%）鸟类对 spp. 呈阳性，4只（0.8%）对 spp. 呈阳性；此外，在靶向16S rRNA基因的PCR中，13只（2.6%）对无形体科病原体呈阳性。在、和中检测到的16S rRNA序列与 sp. Magellanica密切相关。在和中检测到的序列与分组。在和中检测到的16S rRNA基因型与Allocryptoplasma分组。在、和中检测到的23S - 5S基因型与相关。总之，在潘塔纳尔湿地的鸟类中检测到了、和Allocryptoplasma的新基因型。