Appegren Anaïs, Boschiroli Maria Laura, De Cruz Krystel, Michelet Lorraine, Héry-Arnaud Geneviève, Kempf Marie, Lanotte Philippe, Bemer Pascale, Peuchant Olivia, Pestel-Caron Martine, Skalli Soumaya, Brasme Lucien, Martin Christian, Enault Cecilia, Carricajo Anne, Guet-Revillet Hélène, Ponsoda Michaël, Jacomo Véronique, Bourgoin Anne, Trombert-Paolantoni Sabine, Carrière Christian, Dupont Chloé, Conquet Guilhem, Galal Lokman, Banuls Anne-Laure, Godreuil Sylvain
Laboratory of Bacteriology, CHU Montpellier, 34000 Montpellier, France.
ANSES Laboratory for Animal Health, Tuberculosis National Reference Laboratory, University Paris-Est, 94000 Maisons-Alfort, France.
Pathogens. 2023 Apr 1;12(4):548. doi: 10.3390/pathogens12040548.
infects cattle and wildlife, and also causes a small proportion of tuberculosis cases in humans. In most European countries, infections in cattle have been drastically reduced, but not eradicated. Here, to determine the circulation within and between the human, cattle, and wildlife compartments, we characterized by spoligotyping and mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing the genetic diversity of isolates collected from humans, cattle, and wildlife in France from 2000 to 2010. We also assessed their genetic structure within and among the different host groups, and across time and space. The genetic structure and its spatiotemporal variations showed different dynamics in the human and animal compartments. Most genotypes detected in human isolates were absent in cattle and wildlife isolates, possibly because in patients, infection was contracted abroad or was the reactivation of an old lesion. Therefore, they did not match the genetic pool present in France during the study period. However, some human-cattle exchanges occurred because some genotypes were common to both compartments. This study provides new elements for understanding epidemiology in France, and calls for increased efforts to control this pathogen worldwide.
它感染牛和野生动物，也导致一小部分人类结核病病例。在大多数欧洲国家，牛的感染已大幅减少，但尚未根除。在此，为了确定人类、牛和野生动物群体内部以及它们之间的传播情况，我们通过间隔寡核苷酸分型（spoligotyping）和分枝杆菌插入重复单位可变数目串联重复序列（MIRU-VNTR）分型，对2000年至2010年从法国的人类、牛和野生动物中分离出的菌株的遗传多样性进行了表征。我们还评估了它们在不同宿主群体内部和之间、以及跨越时间和空间的遗传结构。遗传结构及其时空变化在人类和动物群体中表现出不同的动态。在人类分离株中检测到的大多数基因型在牛和野生动物分离株中不存在，这可能是因为在患者中，感染是在国外感染的，或者是旧病灶的重新激活。因此，它们与研究期间法国存在的基因库不匹配。然而，由于一些基因型在两个群体中都很常见，所以发生了一些人类与牛之间的传播。这项研究为理解法国的结核病流行病学提供了新的要素，并呼吁在全球范围内加大对这种病原体的控制力度。
