Radulski Łukasz, Krajewska-Wędzina Monika, Lipiec Marek, Weiner Marcin, Zabost Anna, Augustynowicz-Kopeć Ewa
County Veterinary Inspectorate in Puławy, C.K. Norwida 17, 24-100 Puławy, Poland.
National Veterinary Research Institute, 24-100 Puławy, Poland.
Pathogens. 2023 Apr 7;12(4):570. doi: 10.3390/pathogens12040570.
Over the last 30 years, the number of invasive turtle species living in the wild has significantly increased in Poland. This proliferation carries many threats, which mainly include the displacement of native species of animals from their natural habitats. Turtles can also be reservoirs for pathogens, including bacteria from the genus. In order to confirm or rule out the presence of acid-fast mycobacteria in the population of invasive turtle species, samples from carapace, plastron, internal organs and mouth cavity swabs from 125 animals were tested. Twenty-eight mycobacterial strains were isolated in culture, which were classified as atypical following multiplex-PCR reactions. The GenoType Mycobacterium Common Mycobacteria (CM) test, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, PCR-restriction fragment length polymorphism (PRA)- and DNA sequencing were used to identify the species of isolates. Of the 28 strains, 11 were identified as , 10 as , 3 as ssp. , 2 as and 1 each of and . The results of the research will also strengthen the understanding that these animals can be vectors for pathogens when living in the wild.
在过去30年里，波兰野生入侵龟类的数量显著增加。这种繁殖带来了许多威胁，主要包括本地动物物种被赶出其自然栖息地。龟类也可能是病原体的宿主，包括该属的细菌。为了确认或排除入侵龟类种群中是否存在抗酸分枝杆菌，对125只龟的背甲、腹甲、内脏和口腔拭子样本进行了检测。培养物中分离出28株分枝杆菌菌株，经多重聚合酶链反应（multiplex-PCR）反应后被归类为非典型菌株。使用基因分型分枝杆菌通用分枝杆菌（CM）检测、基质辅助激光解吸/电离飞行时间质谱、聚合酶链反应-限制性片段长度多态性（PRA）和DNA测序来鉴定分离菌株的种类。在这28株菌株中，11株被鉴定为 ，10株为 ，3株为 亚种，2株为 ，1株为 和1株为 。研究结果还将加深人们对这些动物在野外生活时可能成为病原体载体的理解。
