Milavetz B I, Edwards D R
J Cell Physiol. 1986 Jun;127(3):388-96. doi: 10.1002/jcp.1041270306.
The major [35S]methionine-radiolabeled nuclear matrix proteins of mouse 3T3 cells were isolated, and most of these were found to be similar in molecular weight, charge, and protease fingerprint to the nuclear matrix proteins of African green monkey kidney cells, which are found tightly bound to simian virus 40 chromosomes. These nuclear matrix proteins were found to be synthesized in quiescent and serum-stimulated cells and to be turned over slowly during pulse-chase experiments. In contrast, a 70-Kd (kilodalton) neutral protein identified as lamin a was found to be turned over rapidly, producing a 68-Kd protein with a similar isoelectric point. In addition, we observed a decrease in the amounts of two chromatin-bound matrix proteins and a relative increase in lamin a following labeling in the presence of 2 micrograms/ml actinomycin D. However, these effects do not appear to be a result of inhibition of transcription, since they were not observed with other inhibitors (alpha-amanitin and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole).
从小鼠3T3细胞中分离出主要的[35S]甲硫氨酸放射性标记的核基质蛋白，发现其中大多数在分子量、电荷和蛋白酶指纹图谱方面与非洲绿猴肾细胞的核基质蛋白相似，后者紧密结合于猿猴病毒40染色体。这些核基质蛋白在静止细胞和血清刺激的细胞中均有合成，并且在脉冲追踪实验中周转缓慢。相比之下，一种被鉴定为核纤层蛋白A的70千道尔顿中性蛋白周转迅速，产生一种等电点相似的68千道尔顿蛋白。此外，我们观察到在存在2微克/毫升放线菌素D的情况下进行标记后，两种与染色质结合的基质蛋白的量减少，而核纤层蛋白A相对增加。然而，这些效应似乎不是转录抑制的结果，因为在使用其他抑制剂（α-鹅膏蕈碱和5,6-二氯-1-β-D-呋喃核糖基苯并咪唑）时未观察到这些效应。
