Starov A I, Bukrinskaia A G
Mol Biol (Mosk). 1981 Nov-Dec;15(6):1415-22.
The synthesis of the complete and incomplete transcripts (the templates in genome replication and mRNA, respectively) of influenza A WSN virus in chicken fibroblasts was analyzed by gel electrophoresis analysis of the duplexes formed between virion RNA and complementary RNA. Three steps in the transcription could be defined: 1) primary transcription when similar amounts of mRNA of all the genes are accumulated; 2) early secondary transcription when mRNA of NS gene is synthesized in larger amounts than that of other genes and 3) late secondary transcription when the amplification of transcription from all the genes is performed. The synthesis of complete transcripts starts during or after primary transcription. When actinomycin D was added to infected cells, the synthesis of incomplete transcripts was inhibited to a larger degree then that of complete transcripts. Most of incomplete transcripts was observed within the cell nucleic while the complete transcripts were found in the nuclei and cytoplasm, suggesting that the synthesis of incomplete transcripts is located in the nuclei. alpha-Amanitin blocked the synthesis of incomplete transcripts without interfering with that of complete transcripts. These data suggest that the synthesis of complete transcripts does not require the synthesis of cell mRNA as primers in transcription.
通过对甲型流感病毒WSN株的病毒粒子RNA与互补RNA形成的双链体进行凝胶电泳分析，研究了其在鸡成纤维细胞中完整和不完整转录本（分别为基因组复制和mRNA的模板）的合成情况。转录过程可分为三个阶段：1）初级转录阶段，所有基因的mRNA积累量相似；2）早期次级转录阶段，NS基因的mRNA合成量多于其他基因；3）晚期次级转录阶段，所有基因的转录均得到扩增。完整转录本的合成在初级转录阶段或之后开始。当向感染细胞中加入放线菌素D时，不完整转录本的合成受到的抑制程度大于完整转录本。大部分不完整转录本存在于细胞核内，而完整转录本则存在于细胞核和细胞质中，这表明不完整转录本的合成位于细胞核内。α-鹅膏蕈碱可阻断不完整转录本的合成，而不影响完整转录本的合成。这些数据表明，完整转录本的合成在转录过程中不需要细胞mRNA的合成作为引物。
