Massaldi H A, Richieri G V, Mel H C
J Cell Physiol. 1986 Jun;127(3):448-50. doi: 10.1002/jcp.1041270314.
In a recent publication, Heubusch et al. (J Cell. Physiol, 122:266-272, 1985) reported changes of erythrocyte volume measured by the Coulter counter technique over a wide range of osmolalities (160 to 3000 m0sm). Their results showed a partially hindered, nonlinear response, in contrast to classical observations made over more restricted osmolality ranges, using other methods. The authors suggested the underlying cause of this behavior to be a mechanical resistance of the membrane cytoskeleton. In this paper, we wish to offer a different interpretation of their results on erythrocyte osmotic behavior, based on similar experiments carried out in our laboratory, and supported by previous analyses from the literature. In particular, it is shown that the shape-factor correction to the electronic sizing measurement can correctly account for the observed deviations from linearity in the hypotonic range. In contrast, increased chemical nonideality and eventual hemolysis are the likely factors responsible for the behavior in the hypertonic range.
在最近的一篇出版物中，休布施等人（《细胞生理学杂志》，122:266 - 272，1985年）报告了通过库尔特计数器技术在很宽的渗透压范围（160至3000毫渗量）内测量的红细胞体积变化。他们的结果显示出部分受阻的非线性反应，这与使用其他方法在更有限的渗透压范围内进行的经典观察结果形成对比。作者认为这种行为的潜在原因是膜细胞骨架的机械阻力。在本文中，基于我们实验室进行的类似实验，并得到文献先前分析的支持，我们希望对他们关于红细胞渗透行为的结果给出不同的解释。特别是，结果表明对电子尺寸测量的形状因子校正能够正确解释在低渗范围内观察到的与线性的偏差。相比之下，化学非理想性增加和最终的溶血可能是高渗范围内行为的影响因素。
