使用高效液相色谱法对心肌中的主要高能化合物及其分解代谢产物进行简单的梯度洗脱。
Simple step gradient elution of the major high-energy compounds and their catabolites in cardiac muscle using high-performance liquid chromatography.
作者信息
Hull-Ryde E A, Lewis W R, Veronee C D, Lowe J E
出版信息
J Chromatogr. 1986 Apr 25;377:165-74. doi: 10.1016/s0378-4347(00)80771-9.
The majority of high-energy nucleotides and their catabolites are separated in a single 13-min run using reversed-phase high-performance liquid chromatography. Economical step gradient elution of these compounds with a Nova Pak-A C18, 5-microns, 10 cm X 8 mm column accomplishes this separation with recoveries of 94-100% and sensitivities of 1-5 pmol. Furthermore, the total adenine nucleotide pool can now be quantitated precisely in a simple and easily automated procedure.
使用反相高效液相色谱法，在13分钟的单次运行中可分离出大多数高能核苷酸及其分解代谢产物。使用Nova Pak-A C18、5微米、10厘米×8毫米的色谱柱对这些化合物进行经济的梯度洗脱，可实现这种分离，回收率为94%-100%，灵敏度为1-5皮摩尔。此外，现在可以通过一个简单且易于自动化的程序精确测定总腺嘌呤核苷酸池。
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