Simultaneous determination of creatine compounds and adenine nucleotides in myocardial tissue by high-performance liquid chromatography.

A rapid high-performance liquid chromatography method was developed for the determination of creatine phosphate, creatine, adenine nucleotides, and related compounds in myocardial tissue. Analysis was performed by reversed-phase chromatography on a C18 column containing 3-microns particles, employing gradient elution and uv detection at 210 nm. Separation was achieved in less than 5 min. Total analysis time, including equilibration of the column after return of the gradient to starting conditions, was 8 min. The high reproducibility and short analysis time make this method suitable for the routine analysis of large series of samples.