采用毛细管气相色谱-质谱联用法定量分析血浆中的1-(2-嘧啶基)哌嗪。
Quantitative analysis of 1-(2-pyrimidinyl)piperazine in plasma by capillary gas chromatography-mass spectrometry.
作者信息
Kerns E H, Bullen W W, Gammans R E
出版信息
J Chromatogr. 1986 Apr 25;377:195-203. doi: 10.1016/s0378-4347(00)80774-4.
A sensitive method for detecting and quantifying 1-(2-pyrimidinyl)piperazine, an important metabolite of buspirone, in human plasma was developed and validated. The range of the method is 0.2-15 ng/ml. The analyte was removed from buffered, sodium chloride-saturated plasma with benzene, extracted into aqueous acid, washed with diethyl ether and reextracted into benzene. The processed extract was derivatized with pentafluorobenzoyl chloride. Instrumental analysis involved on-column injection into a fused-silica capillary gas chromatography column and detection by selected-ion monitoring mass spectrometry.
开发并验证了一种灵敏的方法，用于检测和定量人血浆中丁螺环酮的重要代谢物1-(2-嘧啶基)哌嗪。该方法的线性范围为0.2-15 ng/ml。将分析物从缓冲的、饱和氯化钠血浆中用苯去除，萃取到酸性水溶液中，用乙醚洗涤，再萃取到苯中。处理后的萃取物用五氟苯甲酰氯衍生化。仪器分析包括柱上进样到熔融石英毛细管气相色谱柱中，并通过选择离子监测质谱进行检测。
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