Becker R, Lintz W
J Chromatogr. 1986 Apr 25;377:213-20. doi: 10.1016/s0378-4347(00)80776-8.
A specific, sensitive and precise method for the determination of tramadol in human serum is described. It comprises a three-step extraction procedure and a specific determination by capillary gas chromatography with nitrogen-selective detection, using a homologue of tramadol as an internal standard. The specificity of the method was checked by gas chromatography-mass spectrometry. Precision parameters were 1.7-5.5% (within-run) and 3.2-5.7% (between-run) in the concentration range 12.5-200 ng/ml. The detection limit was about 3 ng/ml.
本文描述了一种测定人血清中曲马多的特异、灵敏且精确的方法。该方法包括三步萃取程序，以及使用曲马多的同系物作为内标，通过氮选择性检测的毛细管气相色谱法进行特异性测定。通过气相色谱-质谱联用仪对该方法的特异性进行了检验。在12.5-200 ng/ml的浓度范围内，精密度参数为批内1.7-5.5%，批间3.2-5.7%。检测限约为3 ng/ml。
