Huber-Smith M J, Nesse R, Mazhar M, McCann D S
J Chromatogr. 1986 Apr 25;377:91-9. doi: 10.1016/s0378-4347(00)80764-1.
A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [3H]MHPG. The HPLC procedure utilizes a C18 column, isocratic elution and amperometric detection. The assay was checked against a gas chromatographic-mass spectrometric procedure; the two procedures correlated well with a correlation coefficient of 0.99. Intra-assay reproducibility was 5.4%, inter-assay reproducibility 10.7%. Immediate changes in the orthostatic position did not affect the plasma MHPG concentration. Based on 22 normal controls the daytime plasma MHPG level was 2.98 +/- 0.66 ng/ml (mean +/- S.D.).
本文介绍了一种适用于评估1毫升血浆样本中3-甲氧基-4-羟基苯乙二醇（MHPG）的高效液相色谱（HPLC）测定法。预处理步骤包括用乙酸乙酯萃取和微型柱色谱法。用[³H]MHPG监测回收率。HPLC程序采用C18柱、等度洗脱和安培检测。该测定法与气相色谱-质谱法进行了对照检查；两种方法相关性良好，相关系数为0.99。批内重复性为5.4%，批间重复性为10.7%。体位立即改变不影响血浆MHPG浓度。基于22名正常对照者，白天血浆MHPG水平为2.98±0.66纳克/毫升（平均值±标准差）。
