Minegishi A, Ishizaki T
J Chromatogr. 1984 Nov 9;311(1):51-7. doi: 10.1016/s0378-4347(00)84690-3.
A reversed-phase liquid chromatographic method with amperometric detection has been developed for determining free 3-methoxy-4-hydroxyphenylglycol in plasma. The method is based on a simple and rapid extraction procedure employing a small C18 column. Vanillyl alcohol was used as an internal standard to obtain a good reproducibility. The 3-methoxy-4-hydroxyphenylglycol concentrations measured with the present method were in reasonable agreement with recently published data using high-performance liquid chromatography with amperometric detection and gas chromatography with mass spectrometry. The additional advantage of the present assay is that it can be performed in parallel with the quantification of other monoamine metabolites in plasma.
已开发出一种采用安培检测的反相液相色谱法，用于测定血浆中的游离3-甲氧基-4-羟基苯乙二醇。该方法基于使用小C18柱的简单快速萃取程序。使用香草醇作为内标以获得良好的重现性。用本方法测得的3-甲氧基-4-羟基苯乙二醇浓度与最近发表的使用高效液相色谱-安培检测和气相色谱-质谱法的数据合理一致。本测定法的另一个优点是它可以与血浆中其他单胺代谢物的定量分析同时进行。
