Eom Heonseop
Department of Civil Engineering, Keimyung University, 1095 Dalgubeol-daero, Dalseo-gu, Daegu 42601, Republic of Korea.
Toxics. 2023 Apr 8;11(4):352. doi: 10.3390/toxics11040352.
Microbial toxicity tests are considered efficient screening tools for the assessment of water contamination. The objective of this study was to develop a sulfur-oxidizing bacteria (SOB)-based ecotoxicity test with high sensitivity and reproducibility for simple and rapid on-site application. To attain this goal, we developed a 25 mL vial-based toxicity kit and improved our earlier SOB toxicity test technique. The current study applied a suspended form of SOB and shortened the processing time to 30 min. Moreover, we optimized the test conditions of the SOB toxicity kit in terms of initial cell density, incubating temperature, and mixing intensity during incubation. We determined that 2 × 10 cells/mL initial cell density, 32 °C incubating temperature, and 120 rpm mixing intensity are the optimal test conditions. Using these test conditions, we performed SOB toxicity tests for heavy metals and petrochemicals, and obtained better detection sensitivity and test reproducibility, compared to earlier SOB tests. Our SOB toxicity kit tests have numerous advantages, including a straightforward test protocol, no requirement of sophisticated laboratory equipment, and no distortion of test results from false readings of end-points and properties of test samples, making it suitable for simple and rapid on-site application.
微生物毒性测试被认为是评估水污染的有效筛选工具。本研究的目的是开发一种基于硫氧化细菌（SOB）的生态毒性测试方法，该方法具有高灵敏度和可重复性，以便于简单快速地现场应用。为实现这一目标，我们开发了一种基于25 mL小瓶的毒性检测试剂盒，并改进了我们早期的SOB毒性测试技术。当前的研究采用了悬浮形式的SOB，并将处理时间缩短至30分钟。此外，我们在初始细胞密度、孵育温度和孵育期间的混合强度方面优化了SOB毒性检测试剂盒的测试条件。我们确定初始细胞密度为2×10个细胞/mL、孵育温度为32°C和混合强度为120 rpm是最佳测试条件。使用这些测试条件，我们对重金属和石化产品进行了SOB毒性测试，与早期的SOB测试相比，获得了更好的检测灵敏度和测试可重复性。我们的SOB毒性检测试剂盒测试有许多优点，包括测试方案简单、不需要复杂的实验室设备，以及不会因测试样品终点和性质的错误读数而导致测试结果失真，使其适用于简单快速的现场应用。
