从居住在埃塞俄比亚的难民中分离出的[物质]的分子分型和药敏谱。 需注意，原文中“isolated from...”前缺少具体所指物质，翻译时补充了“[物质]”以使译文更通顺完整。
Molecular typing and drug sensitivity profiles of isolated from refugees residing in Ethiopia.
作者信息
Meaza Abyot, Diriba Getu, Girma Musse, Wondimu Ammanuel, Worku Getnet, Medhin Girmay, Ameni Gobena, Gumi Balako
机构信息
Aklilu Lemma Institute of Pathobiology (ALIPB), Addis Ababa University (AAU), P.O. Box 1176, Addis Ababa, Ethiopia.
Ethiopian Public Health Institute (EPHI), PO Box 1242, Swaziland Street, Addis Ababa, Ethiopia.
出版信息
J Clin Tuberc Other Mycobact Dis. 2023 Apr 15;31:100371. doi: 10.1016/j.jctube.2023.100371. eCollection 2023 May.
BACKGROUND
Refugees in developing countries have poor access to Tuberculosis (TB) care and control services. The understanding of genetic diversity and drug sensitivity patterns of (MTB) is important for the TB control program. However, there is no evidence that shows the drug sensitivity profiles and genetic diversity of MTB circulating among refugees residing in Ethiopia. This study aimed to investigate the genetic diversity of MTB strains and lineages, and to identify the drug sensitivity profiles of MTB isolated from refugees residing in Ethiopia.
METHODS
A cross-sectional study was conducted among 68 MTB positive cases isolated from presumptive TB refugees from February to August 2021. Data and samples were collected in the refugee camp clinics and both rapid TB Ag detection and region of difference (RD)-9 deletion typing were used to confirm the MTBs. Drug susceptibility test (DST) and molecular typing were done using Mycobacterium Growth Indicator Tube (MGIT) method and spoligotyping respectively.
RESULTS
DST and spoligotyping results were available for all 68 isolates. The isolates were grouped into 25 spoligotype patterns, which consisted of 1-31 isolates with 36.8% strain diversity. The international shared type (SIT)25 was predominant spoligotype pattern consisting of 31 (45.6%) isolates, followed by SIT24 comprising 5 (7.4%) isolates. Further investigation showed that 64.7% (44/68) of the isolates were belonged to CAS1-Delhi family and 75% (51/68) of the isolates were belonged to lineage(L)-3. Multi-drug resistance (MDR)-TB was observed only in one isolate (1.5%) for first-line anti-TB drugs and the highest level of mono-resistance, 5.9% (4/68), was observed for PZA(Pyrazinamide). Mono-resistance was observed in 2.9 % (2/68) and while 97.0% (66/68) of the MTB positive cases were susceptible to the second-line anti-TB drugs.
CONCLUSION
The findings are useful evidence for the TB screening, treatment and control in refugee populations and surrounding communities in Ethiopia.
背景
发展中国家的难民难以获得结核病（TB）的治疗和控制服务。了解结核分枝杆菌（MTB）的基因多样性和药敏模式对于结核病控制项目很重要。然而，没有证据表明埃塞俄比亚境内难民中传播的MTB的药敏谱和基因多样性情况。本研究旨在调查埃塞俄比亚境内难民中MTB菌株和谱系的基因多样性，并确定分离出的MTB的药敏谱。
方法
对2021年2月至8月从疑似结核病难民中分离出的68例MTB阳性病例进行了横断面研究。在难民营诊所收集数据和样本，采用快速结核抗原检测和差异区域（RD）-9缺失分型来确认MTB。分别使用分枝杆菌生长指示管（MGIT）方法和间隔寡核苷酸分型法进行药敏试验（DST）和分子分型。
结果
所有68株分离株均获得了DST和间隔寡核苷酸分型结果。这些分离株被分为25种间隔寡核苷酸分型模式，其中包含1至31株分离株，菌株多样性为36.8%。国际共享型（SIT）25是主要的间隔寡核苷酸分型模式，由31株（45.6%）分离株组成，其次是SIT24，包含5株（7.4%）分离株。进一步调查显示，64.7%（44/68）的分离株属于CAS1-德里家族，75%（51/68）的分离株属于谱系（L）-3。仅在1株（1.5%）分离株中观察到对一线抗结核药物的耐多药（MDR）-TB，对吡嗪酰胺（PZA）的单耐药率最高，为5.9%（4/68）。观察到单耐药率为2.9%（2/68），而97.0%（66/68）的MTB阳性病例对二线抗结核药物敏感。
结论
这些发现为埃塞俄比亚难民群体及周边社区的结核病筛查、治疗和控制提供了有用的证据。
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