使用间隔寡核苷酸分型法对苏丹肺部分离株进行基因分型
Genotyping of Pulmonary Isolates from Sudan Using Spoligotyping.
作者信息
Eldirdery Muataz M, Alrayah Intisar E, ElkareIm Mona Oa Awad, Khalid Fatima A, Elegail Asrar M A/Salam, Ibrahim Nuha Y, Nour Eman O M, Ali Rahma H, Hailu Elena, Abebe Markos, Aseffa Abraham, Osman Najem Aldin M, Mukhtar Maowia M, Elhaj Nihad M A, Elagib Atif A
机构信息
Tropical Medicine Research Institute, National Center for Research, Khartoum, Sudan.
College of Applied Medical Science, Shaqra University, Shaqra, KSA.
出版信息
Am J Microbiol Res. 2015;3(4):125-128. doi: 10.12691/ajmr-3-4-1.
Tuberculosis (TB) remains a major public health problem worldwide due to its high risk of person-to-person transmission, morbidity and mortality [1]. Sudan has a high burden of tuberculosis. Spoligotyping (spacer oligonucleotide typing) a rapid method for genotyping of using the principle of reverse hybridization. The ecology of the prevalent mycobacteria strain can vary depending on country and region. The aim of this study was to determine the genotyping of isolated from Sudan using spoligotyping SPOLDB4. A total of 75 sputum samples were collected from pulmonary Tuberculosis patients attending references Laboratories and diagnostic centers in Khartoum and Eastern Sudan in (2011-2013). The mycobacteria were genotyped using Spoligotyping technique and data obtained were analyzed and compared to the SPOLDB4 database. Among the 75 isolate analyzed, 57(76%) were identified by SPOLDB4 and 18 (24%) could not be matched to any lineages. The most prevalent genotype cluster was MANU2 38 (50.7%) followed by CASI Delhi 8 (10.7%). In the study SIT54 was the most common pattern 37 (49.3%) followed by SIT25 6(8%).
由于结核病（TB）在人与人之间传播、发病和死亡的风险很高，它仍然是全球主要的公共卫生问题[1]。苏丹的结核病负担很重。间隔寡核苷酸分型（Spoligotyping）是一种基于反向杂交原理的快速基因分型方法。流行分枝杆菌菌株的生态可能因国家和地区而异。本研究的目的是使用Spoligotyping SPOLDB4确定从苏丹分离的结核分枝杆菌的基因分型。2011年至2013年期间，从喀土穆和苏丹东部的参考实验室及诊断中心的肺结核患者中总共收集了75份痰标本。使用间隔寡核苷酸分型技术对分枝杆菌进行基因分型，并对获得的数据进行分析，并与SPOLDB4数据库进行比较。在分析的75株分离株中，57株（76%）被SPOLDB4鉴定出来，18株（24%）无法与任何谱系匹配。最常见的基因型簇是MANU2 38株（50.7%），其次是CASI Delhi 8株（10.7%）。在该研究中，SIT54是最常见的模式37株（49.3%），其次是SIT25 6株（8%）。
Zotero 插件