埃塞俄比亚结核分枝杆菌 spoligotyping 基因多样性的系统评价。
Spoligotyping based genetic diversity of Mycobacterium tuberculosis in Ethiopia: a systematic review.
机构信息
Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P. O. Box 1176, Addis Ababa, Ethiopia.
Department of Microbiology, Immunology and Parasitology, Bahir Dar University, P. O. Box 79, Bahir Dar, Ethiopia.
出版信息
BMC Infect Dis. 2018 Mar 27;18(1):140. doi: 10.1186/s12879-018-3046-4.
BACKGROUND
Understanding the types of strains and lineages of Mycobacterium tuberculosis (M. tuberculosis) circulating in a country is of paramount importance for tuberculosis (TB) control program of that country. The main aim of this study was to review and compile the results of studies conducted on strains and lineages of M. tuberculosis in Ethiopia.
METHODS
A systematic search and review of articles published on M. tuberculosis strains and lineages in Ethiopia were made. PubMed and Google Scholar databases were considered for the search while the keywords used were M. tuberculosis, molecular epidemiology, molecular typing spoligotyping and Ethiopia.
RESULT
Twenty-one studies were considered in this review and a total of 3071 M. tuberculosis isolates and 3067 strains were included. These studies used spoligotyping and identified five lineages including Indo-Ocean, East Asian/Beijing, East African-Indian, Euro-American and Ethiopian in a proportion of 7.1%, 0.2%, 23.0%, 64.8%, and 4.1%, respectively. Thus, Euro-American was the most frequently (64.8%) occurring Lineage while East Asian was the least (0.2%) frequently occurring Lineage in the country. Surprisingly, the Ethiopian Lineage seemed to be localized to northeastern Ethiopia. In addition, the top five clades identified by this review were T, CAS, H, Manu and Ethiopian comprising of 48.0%, 23.0%, 11.0%, 6.0% and 4.1% of the strains, respectively. Furthermore, predominant shared types (spoligotype patterns) identified were SIT149, SIT53, SIT25, SIT37, and SIT21, each consisting of 420, 343, 266, 162 and 102 isolates, respectively, while, on the other hand, 15% of the strains were orphan.
CONCLUSION
According to the summary of the results of this review, diversified strains and lineages of M. tuberculosis were found in Ethiopia, and the frequencies of occurrence of these strains and lineages were variable in different regions of the country. This systematic review is registered in the PRISMA with the registration number of 42017059263.
背景
了解在一个国家中循环的结核分枝杆菌（Mycobacterium tuberculosis，M. tuberculosis）菌株和谱系类型对于该国家的结核病（tuberculosis，TB）控制计划至关重要。本研究的主要目的是回顾和汇编在埃塞俄比亚进行的关于 M. tuberculosis 菌株和谱系的研究结果。
方法
对在埃塞俄比亚发表的关于 M. tuberculosis 菌株和谱系的文章进行了系统搜索和综述。考虑使用 PubMed 和 Google Scholar 数据库进行搜索，使用的关键词是 M. tuberculosis、分子流行病学、分子分型 spoligotyping 和埃塞俄比亚。
结果
本综述考虑了 21 项研究，共纳入 3071 株 M. tuberculosis 分离株和 3067 株菌株。这些研究使用 spoligotyping 鉴定了五个谱系，包括印度-印度洋、东亚/北京、东非-印度、欧亚和埃塞俄比亚谱系，比例分别为 7.1%、0.2%、23.0%、64.8%和 4.1%。因此，欧亚谱系是该国最常见（64.8%）的谱系，而东亚谱系是最不常见（0.2%）的谱系。令人惊讶的是，埃塞俄比亚谱系似乎局限于埃塞俄比亚东北部。此外，本综述确定的前五个分支是 T、CAS、H、Manu 和埃塞俄比亚分支，分别占菌株的 48.0%、23.0%、11.0%、6.0%和 4.1%。此外，鉴定出的主要共享类型（ spoligotype 模式）是 SIT149、SIT53、SIT25、SIT37 和 SIT21，每个类型分别包含 420、343、266、162 和 102 株分离株，另一方面，15%的菌株是孤儿株。
结论
根据本综述结果的总结，在埃塞俄比亚发现了多样化的 M. tuberculosis 菌株和谱系，这些菌株和谱系在该国不同地区的发生频率不同。本系统综述已在 PRISMA 中注册，注册号为 42017059263。
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