College of Agricultural Science, Department Animal Science, Veterinary Program, Arba Minch University, P.O. Box: 21, Arba Minch, Ethiopia.
College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia.
Trop Anim Health Prod. 2023 Apr 28;55(3):177. doi: 10.1007/s11250-023-03593-5.
This study was aimed to assess the performance of Trichostrongylus colubriformis and the host's responses to regulate egg outputs and worm burden. Infective larva (L3) was prepared by culturing egg of the worms harvested from the intestine of slaughtered sheep. Following this, L3 was maintained in the donor sheep to get adequate quantity of it for experimental trials. A complete randomized block design was used by considering host as blocking factor. Twenty eight small ruminants (sheep = 14) and (goat = 14) were employed in such a way that half of them were drenched with 10,000 T. colubriformis L3 and the remaining halves were controls. Faecal egg count (FEC) was recorded from the initial period (day-zero) up to day-56. At the end of experiment, animals were euthanized in humane manner, worms were recovered from the intestines, counted, and burden was estimated. FEC at various days post infection was insignificantly (P > 0.05) higher in goats than in sheep. The worm burden was significantly (P = 0.040) higher in infected goat than in infected sheep despite they were given equal dose of L3. In conclusion, relatively lower worm burden under natural conditions in goat may be due to their feeding behavior rather than their inherent resistance.
本研究旨在评估卷曲毛线虫的性能以及宿主对调节产卵量和蠕虫负担的反应。通过培养从屠宰羊肠道中收获的蠕虫卵来制备感染性幼虫 (L3)。此后，L3 在供体羊中维持，以获得足够数量的 L3 用于实验。采用完全随机区组设计，以宿主为区组因素。使用 28 只小反刍动物（绵羊=14 只，山羊=14 只），其中一半用 10,000 条卷曲毛线虫 L3 进行喷淋，另一半为对照组。从初始阶段（第 0 天）到第 56 天记录粪便卵计数（FEC）。实验结束时，以人道方式对动物进行安乐死，从肠道中回收、计数蠕虫，并估计负担。感染后不同天数的 FEC 在山羊中显著（P > 0.05）高于绵羊。尽管给予了等量的 L3，但感染山羊的蠕虫负担明显（P = 0.040）高于感染绵羊。在自然条件下，山羊的蠕虫负担相对较低可能是由于它们的进食行为而不是内在的抵抗力。
