Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, P. R. China.
Analyst. 2019 Nov 7;144(21):6432-6437. doi: 10.1039/c9an01600g. Epub 2019 Oct 4.
An electrochemical platform was proposed for highly sensitive and selective analysis of sialic acid based on molecularly imprinted polymers (MIPs), which were electropolymerized with the monomer molecules of 3-aminophenylboronic acid (ABA) on a carbon cloth (CC) electrode in the presence of template molecules. The fabricated sensor, named the PABA/CC-based MIP electrode, could be used for the detection of sialic acid because the reversible and covalent boronic acid-diol binding was sensitive to the electrochemical potential of the prepared sensor. The utilization of a CC film as the substrate could improve the sensitivity due to its good electrical conductivity and large surface area. Under the optimized conditions, a good relationship between the change in potential and the concentration of sialic acid was obtained in the range from 40 μM to 440 μM with a detection limit of 0.5 μM. The resulting MIP sensor also displayed good selectivity, reproducibility and stability. Moreover, this sensor was successfully applied for the evaluation of the sialic acid level in infant formulas.
电化学平台被提出用于高度敏感和选择性的分析基于唾液酸的分子印迹聚合物（MIPs），该平台是在碳布（CC）电极上在模板分子存在的情况下用单体分子 3-氨苯基硼酸（ABA）电聚合的。所制备的传感器，命名为基于 PABA/CC 的 MIP 电极，可用于检测唾液酸，因为可逆和共价硼酸-二醇键合对制备的传感器的电化学势敏感。使用 CC 膜作为基底可以提高灵敏度，因为它具有良好的导电性和较大的表面积。在优化条件下，在 40 μM 至 440 μM 的范围内，电位变化与唾液酸浓度之间存在良好的关系，检测限为 0.5 μM。所得的 MIP 传感器还表现出良好的选择性、重现性和稳定性。此外，该传感器还成功地应用于婴儿配方奶粉中唾液酸水平的评估。
