Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Jeffrey Cheah Biomedical Centre, Cambridge, CB2 0AW, UK.
Curr Cardiol Rep. 2023 Jun;25(6):495-503. doi: 10.1007/s11886-023-01880-z. Epub 2023 Apr 29.
Formation of the heart requires the coordinated addition of multiple progenitor sources which have undergone different pathways of specification and differentiation. In this review, I aim to put into context how recent studies defining cardiac progenitor heterogeneity build on our understanding of early heart development and also discuss the questions raised by this new insight.
With the development of sequencing technologies and imaging approaches, it has been possible to define, at high temporal resolution, the molecular profile and anatomical location of cardiac progenitors at the single-cell level, during the formation of the mammalian heart. Given the recent progress in our understanding of early heart development and technical advances in high-resolution time-lapse imaging and lineage analysis, we are now in a position of great potential, allowing us to resolve heart formation at previously impossible levels of detail. Understanding how this essential organ forms not only addresses questions of fundamental biological significance but also provides a blueprint for strategies to both treat and model heart disease.
心脏的形成需要多个祖细胞来源的协调添加，这些祖细胞来源经历了不同的特化和分化途径。在这篇综述中，我旨在将最近定义心脏祖细胞异质性的研究置于我们对早期心脏发育的理解的背景下，并讨论这一新见解所提出的问题。
随着测序技术和成像方法的发展，有可能在哺乳动物心脏形成过程中，以高时间分辨率定义心脏祖细胞的分子特征和解剖位置，达到单细胞水平。鉴于我们对早期心脏发育的理解的最新进展以及高分辨率延时成像和谱系分析技术的进步，我们现在处于一个具有巨大潜力的位置，使我们能够以前所未有的细节水平解析心脏形成。了解这个重要器官的形成不仅解决了具有基本生物学意义的问题，而且为治疗和模拟心脏病的策略提供了蓝图。
