De Spiegeleer B, Slegers G, Vandecasteele C, Van den Bossche W, Schelstraete K, Claeys A, De Moerloose P
J Nucl Med. 1986 Mar;27(3):399-403.
A microscale synthesis of [13N]cisplatin (cis-dichlorodiammineplatinum(II), cis-DDP) from cyclotron-produced [13N]ammonia is presented. Temperature, reaction time, ratios, and concentration of reactants have been optimized for each step of the synthesis. Purification is performed by ion exchange chromatography. Radiochemical purity and optimization processes are controlled by high performance liquid chromatography and high performance thin layer chromatography--22 mCi [13N]cisplatin in 10 ml of solution is produced. The entire procedure takes approximately 15 min and the specific activity is approximately 300 mCi/mumole at EOB.
本文介绍了一种从回旋加速器产生的[¹³N]氨微尺度合成[¹³N]顺铂（顺式二氯二氨铂(II)，顺铂-DDP）的方法。合成的每个步骤中，对温度、反应时间、反应物比例和浓度进行了优化。通过离子交换色谱法进行纯化。放射化学纯度和优化过程通过高效液相色谱法和高效薄层色谱法控制——在10毫升溶液中产生了22毫居里的[¹³N]顺铂。整个过程大约需要15分钟，在放化终点时比活度约为300毫居里/微摩尔。
