Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Brazil.
School of Veterinary, State University of Ceará, Fortaleza, Brazil.
Biofouling. 2023 Feb;39(2):218-230. doi: 10.1080/08927014.2023.2202315. Epub 2023 Apr 25.
spp. are emerging opportunistic fungi associated with invasive infections, especially in patients with haematological malignancies. The present study investigated the inhibition of efflux pumps by promethazine (PMZ) as a strategy to control and . Planktonic cells were evaluated for antifungal susceptibility to PMZ, as well as inhibition of efflux. The effect of PMZ was also studied in biofilms. PMZ inhibited and planktonic cells at concentrations ranging from 32 to 256 μgml. Subinhibitory concentrations of PMZ inhibited efflux activity in . Biofilms were completely eradicated by PMZ. PMZ potentiated the action of antifungals, affected the morphology, changed the amount of carbohydrates and proteins and reduced the amount of persister cells inside biofilms. The results showed indirect evidences of the occurrence of efflux pumps in and opens a perspective for the use of this target in the control of trichosporonosis.
种属是与侵袭性感染相关的新兴机会性真菌，尤其是在血液恶性肿瘤患者中。本研究探讨了通过普罗米嗪（PMZ）抑制外排泵作为控制和的策略。评估浮游细胞对 PMZ 的抗真菌敏感性以及外排抑制作用。还研究了 PMZ 在生物膜中的作用。PMZ 以 32 至 256μgml 的浓度抑制和浮游细胞。亚抑制浓度的 PMZ 抑制外排活性。PMZ 可完全消除生物膜中的生物膜。PMZ 增强了抗真菌药物的作用，影响了形态，改变了生物膜中碳水化合物和蛋白质的含量，并减少了持久性细胞的数量。结果表明，外排泵在和中确实存在，为在外排泵控制中的应用提供了新的视角。
