嗜热脂肪芽孢杆菌大核糖体颗粒单晶的表征

Characterization of single crystals of the large ribosomal particles from Bacillus stearothermophilus.

作者信息

Yonath A, Saper M A, Makowski I, Müssig J, Piefke J, Bartunik H D, Bartels K S, Wittmann H G

出版信息

J Mol Biol. 1986 Feb 20;187(4):633-6. doi: 10.1016/0022-2836(86)90342-6.

Abstract

Single, three-dimensional crystals of the 50 S ribosomal subunit from Bacillus stearothermophilus (strain NCA) have been characterized using a synchrotron X-ray source. The crystals are orthorhombic with unit cell dimensions: a = 350 A, b = 670 A, c = 905 A, and contain at least one 2-fold screw axis. With cooling to -2 degrees C, the large crystals (1.0 mm X 0.2 mm X 0.1 mm) diffract to 15 to 18 A resolution and are stable in the synchrotron beam for several hours. Despite the large cell dimensions, the reflections are readily resolved when the X-ray diffraction patterns are densitometered with a 25 microns faster.

摘要

利用同步辐射X射线源对嗜热脂肪芽孢杆菌（菌株NCA）50S核糖体亚基的单晶体三维结构进行了表征。晶体为正交晶系，晶胞参数为：a = 350 Å，b = 670 Å，c = 905 Å，且至少包含一个2次螺旋轴。冷却至-2℃时，大晶体（1.0 mm×0.2 mm×0.1 mm）的衍射分辨率可达15至18 Å，并且在同步辐射束中可稳定存在数小时。尽管晶胞尺寸较大，但当用快25微米的密度计对X射线衍射图谱进行测量时，反射峰仍能清晰分辨。