Homma Y, Oyasu R
J Urol. 1986 Jul;136(1):136-40. doi: 10.1016/s0022-5347(17)44758-6.
The effects of formalin instillation or teflon bead insertion on heterotopically transplanted rat urinary bladders (HTBs) were investigated. A single instillation of 0.3 per cent formalin dissolved in 0.9 per cent NaCl into HTBs induced multifocal superficial ulcers which were quickly repaired by lateral spreading of regenerating cells migrating from the adjacent mucosa. This was associated with a sharp increase in labeling index (10-30 per cent) on days 1 and 2, followed by focal mild (up to five cell layers) simple hyperplasia (SH) on days 4 and 7. Repeated weekly instillation of formalin for 30 weeks induced only focal mild epithelial hyperplasia. Six weekly instillations of urine one day after formalin administration slightly enhanced the formalin-induced rise in labeling index. Insertion of teflon beads caused extensive diffuse remarkable changes, especially in the presence of urine: multifocal to diffuse SH (four to eight cell layers) and multifocal papillary or nodular hyperplasia was observed by six weeks. The results indicate that responses to formalin instillation are acute and transient, while reactions to bead insertion are persistent. In both cases, presence of urine appeared to enhance hyperplastic changes. These models of bladder injury, by inducing two different types of reactions in the bladder, may be useful in examining the role of trauma in bladder carcinogenesis.
研究了福尔马林灌注或聚四氟乙烯珠植入对异位移植大鼠膀胱（HTB）的影响。向HTB中单次灌注溶解于0.9%氯化钠中的0.3%福尔马林会诱导多灶性浅表溃疡，这些溃疡会通过从相邻黏膜迁移而来的再生细胞侧向扩散而迅速修复。这与第1天和第2天标记指数急剧增加（10%-30%）相关，随后在第4天和第7天出现局灶性轻度（最多五层细胞）单纯性增生（SH）。每周重复灌注福尔马林30周仅诱导局灶性轻度上皮增生。在福尔马林给药后一天每周六次灌注尿液略微增强了福尔马林诱导的标记指数升高。聚四氟乙烯珠的植入引起广泛的弥漫性显著变化，尤其是在有尿液存在的情况下：到六周时观察到多灶性至弥漫性SH（四至八层细胞）以及多灶性乳头状或结节状增生。结果表明，对福尔马林灌注的反应是急性和短暂的，而对珠子植入的反应是持久的。在这两种情况下，尿液的存在似乎都会增强增生性变化。这些膀胱损伤模型通过在膀胱中诱导两种不同类型的反应，可能有助于研究创伤在膀胱癌发生中的作用。
