Homma Y, Nowels K, Oyasu R
Cancer Lett. 1986 Aug;32(2):117-23. doi: 10.1016/0304-3835(86)90109-6.
In multistage carcinogenesis, promotion is a long-term or repeated growth stimulation of initiated cells. Possible effects of regenerative hyperplasia induced by repeated intravesical instillation of 0.3% formalin solution on urinary bladder carcinogenesis were examined using heterotopically transplanted rat urinary bladders (HTBs) initiated by N-methyl-N-nitrosourea. The HTB system was chosen because in this system, transient generalized hyperplasia lasting less than a week can be induced readily and repeatedly by intravesical instillation of the formalin solution. No statistically significant tumor enhancement was observed after 15 formalin treatments administered in 30 weeks. It appears that regenerative cytotoxic stimuli, even multiple, may have no significant tumor-promoting activity. Discussed is the possibility that mild and, more importantly, persistent non-cytotoxic stimuli may be more effective as tumor promoters.
在多阶段致癌过程中，促癌是对启动细胞的长期或反复生长刺激。使用经N-甲基-N-亚硝基脲启动的异位移植大鼠膀胱（HTB），研究了0.3%福尔马林溶液反复膀胱内灌注诱导的再生性增生对膀胱癌发生的可能影响。选择HTB系统是因为在该系统中，通过膀胱内灌注福尔马林溶液可轻松且反复诱导持续时间少于一周的短暂全身性增生。在30周内进行15次福尔马林处理后，未观察到统计学上显著的肿瘤增强。看来，即使是多次的再生性细胞毒性刺激，可能也没有显著的促癌活性。文中讨论了温和且更重要的是持续性非细胞毒性刺激作为肿瘤促进剂可能更有效的可能性。
