College of Food Science, Southwest University, Chongqing 400715, China.
College of Food Science and Engineering, Bohai University, Jinzhou 121013, China.
J Agric Food Chem. 2023 May 10;71(18):6920-6934. doi: 10.1021/acs.jafc.2c08559. Epub 2023 Apr 26.
The effect of fish oil (FO) on colonic function, immunity, and microbiota was investigated in (Vp)-infected C57BL/6J mice. Mice intragastrically presupplemented with FO (4.0 mg) significantly reduced Vp infection as evidenced by stabilizing body weight and reducing disease activity index score and immune organ ratios. FO minimized colonic pathological damage, strengthened the mucosal barrier, and sustained epithelial permeability by increasing epithelial crypt depth, goblet cell numbers, and tight junctions and inhibiting colonic collagen accumulation and fibrosis protein expression. Mechanistically, FO enhanced immunity by decreasing colonic CD T cells, increasing CD T cells, downregulating the TLR4 pathway, reducing interleukin-17 (IL-17) and tumor necrosis factor-α, and increasing immune cytokine IL-4 and interferon-γ levels. Additionally, FO maintained colonic microbiota eubiosis by improving microbial diversity and boosting , , and growth and their derived propionic acid and butyric acid levels. Collectively, FO alleviated Vp infection by enriching beneficial colonic microbiota and metabolites and restoring immune homeostasis.
本研究旨在探讨鱼油（FO）对轮状病毒（Vp）感染 C57BL/6J 小鼠结肠功能、免疫和微生物群的影响。结果表明，FO 能稳定体重、降低疾病活动指数评分和免疫器官比值，从而减轻 Vp 感染。FO 还能减轻结肠病理损伤，通过增加肠隐窝深度、杯状细胞数量和紧密连接，抑制结肠胶原积累和纤维化蛋白表达，增强黏膜屏障和维持上皮通透性。机制上，FO 通过减少结肠 CD4+T 细胞、增加 CD8+T 细胞、下调 TLR4 通路、降低白细胞介素-17（IL-17）和肿瘤坏死因子-α（TNF-α），以及增加免疫细胞因子 IL-4 和干扰素-γ（IFN-γ）水平，增强免疫。此外，FO 通过改善微生物多样性，促进有益的结肠微生物生长及其代谢产物丙酸和丁酸的产生，维持结肠微生物群的生态平衡。综上所述，FO 通过丰富有益的结肠微生物群和代谢产物并恢复免疫稳态，缓解 Vp 感染。
